密骨方对去势大鼠骨质疏松后骨折愈合的影响

The effect of migu formula on healing of osteoporotic fracture of ovariectomized rats

目的 观察密骨方对骨质疏松性骨折（OPF）的影响.方法 将90只雌性Wistar大鼠随机分为对照组（Sham）,模型组（OVX）与密骨方治疗组（MGF）,模型组及治疗组行卵巢摘除术,同时MGF组和OVX组大鼠均致其右侧股骨上1/3处骨折.MGF组按100 mg/（kg·d）的剂量灌胃给药,Sham组和OVX组给予同体积的生理盐水,每日1次,疗程分别为2周、4周、6周,用苏木素-伊红（HE）染色检测骨折端的形态学变化;用放射免疫法测定不同时段血清雌二醇（E2）、骨钙素（BGP）的表达;用生化法测定碱性磷酸酶（ALP）的表达.结果 MGF组血清E2在时间上有显著升高趋势（P＜0.05）;MFG组血清BGP值在3个时间段有降低趋势,且差异有统计学意义（P＜0.05）;MFG组血清ALP值总体比较有显著降低趋势（P＜0.05）.结论 MGF有抑制破骨细胞的活性及促进骨折愈合的作用.

Objective To investigate the effect of MGF on healing of Osteoporotic Fracture of ovariectomized rats. Methods 90 female Wistar rats were divided randomly into 3 groups： sham-ovariectomy （SHAM） ,bilateral ovariectomy （OVX） and MGF-treatment groups （MGF）. Perform oophorectomy operations on OVX and MGF-treatment groups. Fractures were created in the 1/3 right femur of OVX and MGF-treatment groups rats. MGF group, 100 mg/（kg·d） of dosage was administered by gavage, while SHAM and OVX groups,the same volumn of sodium chloride were given once a day,the period of treatment was 2 weeks,4 weeks,6 weeks respectively, using HE staing to detect Morphological Changes of fracture ends;The serum E2 and osteocalcin （ BGP） levels were detected by radioimmunoassay;The contents of ALP were detected by chemical and biological methods. Results MGF group serum E2 increased significantly （P 〈 0. 05）; MGF group serum BGP levels descended significantly during these three periods （P 〈 0. 05） ; and overall, MGF group serum ALP levels descended obviously （P 〈 0. 05 ）. Conclusion MGF have an Inhibitory effect on the activity of Osteoclast ang accelerate the union of bone fracture.