基于UPLC指纹图谱相似度的侧柏炭烘制工艺研究

Baking method of Platycladi Cacumen Carbonisatum based on similarity of UPLC fingerprints

目的:建立侧柏炭烘制工艺方法,为中药炭药的炮制工艺研究提供新的思路。方法:采用恒温干燥箱为加热设备,从200℃至250℃间隔10℃共6个温度,每个温度下分别烘制20,30,40 min,共得到18份样品;采用超高效液相色谱法(UPLC),以侧柏炭标准饮片为参照,分析各样品指纹图谱相似度。结果:230℃20 min,230℃30 min,240℃20 min 3份样品的相似度最大,均大于0.96。结论:考虑到指纹图谱相似度,并结合外观性状,确定侧柏炭烘制温度以230℃20 min为宜。

Objective： To establish a baking method of Platycladi Cacumen Carbonisatum for providing a new idea to Carbonic Herbs′ research.Method： Samples were prepared in an oven for different time at different temperatures separately.Then the fingerprints of the samples were determined by UPLC.According to the standard fingerprint,the similarities of the samples′ fingerprints were compared.Result： The similarities of 3 samples,which were baked at 230 ℃ for 20 min,30 min and at 240 ℃ for 20 min,were above 0.96.Conclusion： According to the similarities of the fingerprints and in view of the appearances,Platycladi Cacumen Carbonizing should be baked at 230 ℃ for 20 min.