HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖苷、鸢尾苷、鸢尾苷元的含量

Simultaneous determination of tectorigenin-7-O-xylosylglucosid,tectoridin and tectorigenin in flowers of Pueraria lobata by HPLC

目的：建立HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖苷、鸢尾苷、鸢尾苷元含量的方法。方法：采用Kromasil C18色谱柱（4.6 mm×250 mm,5μm）;流动相为乙腈-0.2%磷酸水溶液采用梯度洗脱,流速1.0 mL.min-1,检测波长265 nm,柱温35℃。结果：鸢尾黄素-7-O-木糖葡萄糖苷、鸢尾苷、鸢尾苷元的线性范围分别为0.14~1.40（r=0.999 4）,0.20~2.02（r=0.999 3）,0.10~1.04μg（r=0.999 1）;平均回收率（n=6）分别为99.5%（RSD 1.8%）,99.6%（RSD 1.7%）,99.8%（RSD 1.9%）。结论：该方法简便、准确,重复性好,为野葛花药材的质量控制提供了实验依据。

Objective： To develop a HPLC method for the simultaneous determination of tectorigenin-7-O-xylosylglucosid,tectoridin and tectorigenin in flowers of Pueraria lobata.Method： The separation was performed on a Kromasil C18 column（4.6 mm×250 mm,5 μm）,using a gradient elution with acetonitrile-water containing 0.2% phosphoric acid as the mobile phase.The flow rate was 1.0 mL·min-1,the detection wavelength was 265 nm and the temperature of column was 35 ℃.Result： The linear ranges of tectorigenin-7-O-xylosylglucosid,tectoridin and tectorigenin were 0.14-1.40（r=0.999 4）,0.20-2.02（r=0.999 3）,and 0.10-1.04（r=0.999 1）,respectively.The average recoveries（n=6） of the three constituents were 99.5%（RSD 1.8%）,99.6%（RSD 1.7%）,and 99.8%（RSD 1.9%）,respectively.Conclusion： The method is simple and accurate with a good eata and can be used as a quality control method for flowers of P.lobata of different sources.