摘要
目的:建立HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖苷、鸢尾苷、鸢尾苷元含量的方法。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.2%磷酸水溶液采用梯度洗脱,流速1.0 mL.min-1,检测波长265 nm,柱温35℃。结果:鸢尾黄素-7-O-木糖葡萄糖苷、鸢尾苷、鸢尾苷元的线性范围分别为0.14~1.40(r=0.999 4),0.20~2.02(r=0.999 3),0.10~1.04μg(r=0.999 1);平均回收率(n=6)分别为99.5%(RSD 1.8%),99.6%(RSD 1.7%),99.8%(RSD 1.9%)。结论:该方法简便、准确,重复性好,为野葛花药材的质量控制提供了实验依据。
Objective: To develop a HPLC method for the simultaneous determination of tectorigenin-7-O-xylosylglucosid,tectoridin and tectorigenin in flowers of Pueraria lobata.Method: The separation was performed on a Kromasil C18 column(4.6 mm×250 mm,5 μm),using a gradient elution with acetonitrile-water containing 0.2% phosphoric acid as the mobile phase.The flow rate was 1.0 mL·min-1,the detection wavelength was 265 nm and the temperature of column was 35 ℃.Result: The linear ranges of tectorigenin-7-O-xylosylglucosid,tectoridin and tectorigenin were 0.14-1.40(r=0.999 4),0.20-2.02(r=0.999 3),and 0.10-1.04(r=0.999 1),respectively.The average recoveries(n=6) of the three constituents were 99.5%(RSD 1.8%),99.6%(RSD 1.7%),and 99.8%(RSD 1.9%),respectively.Conclusion: The method is simple and accurate with a good eata and can be used as a quality control method for flowers of P.lobata of different sources.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第17期2308-2311,共4页
China Journal of Chinese Materia Medica