HPLC测定小花黄堇中原阿片碱的含量

Determination of protopine in Corydalis racemose by HPLC

目的：建立用HPLC测定小花黄堇中原阿片碱含量的方法。方法：Gemini C18色谱柱（4.6 mm×250 mm,5μm）;流动相乙腈-三乙胺醋酸溶液（20∶80）;流速1.0 mL.min-1;检测波长289 nm;柱温30℃。结果：小花黄堇中原阿片碱的平均质量分数0.905%,原阿片碱在0.124~1.36μg线性关系良好,平均回收率98.49%,RSD 1.9%（n=6）。结论：该方法操作简便,重复性较好,测定结果较为可靠,可用于测定小花黄堇中原阿片碱的含量。

Objective： To develop a HPLC method for determining the content of protopine in Corydalis racemose.Method： Analysis was performed on a Gemini C18 column（4.6 mm×250 mm,5 μm） eluted with acetonitrile-water containing 0.8% triethylamine and 3% acetic acid acetum（20∶80） as the mobile phase.The flow rate was 1.0 mL·min-1.The detection wavelength was 289 nm.Result： The average content of protopine in Herb of Racemose Corydalis was 0.905%.The calibration curve of protopine was linear between 0.124-1.36 μg（r=0.999 9）.The average recovery was 98.49% with RSD 1.9%.Conclusion： This method is simple,reproducible and can be used to determine the content of protopine in C.racemose.