高温处理构建不感染内生真菌羽茅种群的方法探讨

Using High-temperature Treatment to Construct Endophyte-free Achnatherum sibiricum

为了寻求构建禾草内生真菌不感染种群的方法,选用羽茅为试验材料,研究了高温处理对内生真菌和宿主活力的影响。结果表明:60℃高温处理种子能有效杀灭种子内的内生真菌,处理时间为30d时,所有检测幼苗均不感染内生真菌。同时高温处理对种子发芽率、发芽势和发芽指数均无显著影响,且在幼苗生长初期对根的生长有促进作用。与其他杀菌方法相比,高温处理的方法具有成本低、技术简单、环境友好等优点,是一种理想的构建羽茅无菌种群的方法。

In this study high temperature（60℃） treatment was used to construct endophyte-free populations of Achnatherum sibiricum（L.） Keng.The results indicated that high temperature treatment was effective in killing the endophyte in the seeds.After 30 days＇ treatment,seedlings were all uninfected.At the same time,high temperature treatment had no adverse effect on germination rate,germinating potential and germination index of treated seeds.On the contrary,high temperature improved initial root growth of the treated seedlings.Compared to other methods,high temperature treatment had some advantages such as cost low,procedure simple and environment friendly.Therefore,this method was an effective way to construct endophyte-free populations of Achnatherum sibiricum.