兴奋性氨基酸及尼莫地平对培养大鼠脑皮层神经元钙电流的影响(英文)

Effects of excitatory amino acids and nimodipine on calcium currents in cultured rat cortical neurons

目的:探讨兴奋性氨基酸(EAA)及钙通道阻断剂对神经元钙通道的影响,方法:用全细胞膜片箝技术研究Bay-K-8644、谷氨酸(Glu)、天门冬氨酸(Asp)及尼莫地平(Nim)对培养大鼠皮层神经元钙电流(I_(Ca))的作用,结果:Bay-K-8644及Glu明显增加I_(Ca) Asp 0.5,5及50 mmol·L^(-1)浓度依赖性地增加I_(Ca)(分别为14.8％,37.1％和53.1％),细胞浴液中加入Nim 10 μmol·L^(-1),I_(Ca)被抑制46.2％,结论:EAA使神经元钙电流增加,Nim抑制神经元的Ca^(2+)内流。

AIM: To study the effect of excitatory amino acid (EAA) and calcium channel blocker on neuronal calcium channels. METHODS: With patch-clamp technique (whole-cell recording), the effects of Bay-K-8644, cesium glutamate, potassium aspartate, and nimodipine ( Nim) on calcium currents ( ICa) in cultured cortical neurons of neonatal rats were studied. RESULTS: ICa was raised obviously by Bay-K-8644 and glutamate. Ica was raised concentration-depend-ently by aspartate (0.5, 5, 50 mmol·L-1), with increasing rates 15 % ± 3 % , 37 %±3 % , and 53 %± 6 %, respectively. The inhibition of ICa was obvious while adding Nun in the bath solution. With Nim 10 μmol· L-1, the inhibitory rate was 46 %± 4 %. CONCLUSION: EAA had increasing effects on neuronal calcium currents and Nim inhibited Ca2 + influx in neurons.