化学修饰型复合汞膜电极测定双黄连口服液中黄芩苷的含量

Determination of baicalin in Shuanghuanglian oral liquid on composite mercury film electrode based on chemical modfied

目的:利用铕离子掺杂普鲁士蓝(europium-doped prussian blue analogue,Eu-PB)化学修饰型复合汞膜电极(GC/Eu-PB/MFE)建立了黄芩苷的测定方法,并探讨了其电化学行为。方法:在BR(pH6.0)支持电解质中,采用差示脉冲伏安法(DPV)和循环伏安法(CV)。结果:GC/Eu-PB/MFE电极与传统玻碳汞膜电极(GC/MFE)相比,同浓度的黄芩苷在GC/Eu-PB/MFE上响应灵敏度高,重复性好。黄芩苷在该电极上表现较强的吸附行为,其还原峰电位为-1.22 V(vs SCE)。还原峰电流与黄芩苷浓度在8.0×10-9～1.0×10-6mol.L-1呈线性关系(r=0.999 2),检测下限为1.2×10?9mol.L-1(S/N=3)。不经预分离直接测定双黄连口服液中黄芩苷的含量,平均回收率为99.83%,平均RSD 2.3%(n=8)。结论:GC/Eu-PB/MFE用于双黄连口服液中黄芩苷含量的测定,检测限低、灵敏、可靠,可用于该制剂的质量控制。

Objective： A novel Eu-PB composite mercury film electrode（GC/Eu-PB/MFE）based on chemical modfied was developed for the determination of baicalin and the investigation of its the electrochemical behavior.Method： Cyclic voltammetry and differential pulse voltammetry were used.Result： The adsorption characteristic of baicalin was observed in Britton-Robinson buffer solution（pH 6.0）at GC/Eu-PB/MFE.The reduction peak of baicalin was observed at-1.22 V（vs SCE）and peak current was linear to the baicalin from 8.0×10^-9 to 1.0×10^-6 mol·L^-1（r=0.999 2,n=8）.The limit of detection was 1.2×10^-9 mol·L^-1（S/N=3）.The average recovery was 99.83%,with RSD of 2.3 %（n=8）.The baicalin in Shuanghuanglian oral can be directly determined without pretreatment.Conclusion： The GC/Eu-PB/MFE was used to determin ebaicalin in Shuanghuanglian oral with good repeatability and high sensitivity.The method can be used for the quality control of Chinese traditional medicines containing Scutellaria baicalensis.