山楂中抑制HMG-CoA还原酶的活性成分及其协同效应的研究

The inhibition activity of chemical constituents in hawthorn fruit and their synergistic action to HMG-CoA reductase

目的:研究山楂降血脂活性成分及作用机制。方法:以HMG-CoA还原酶活性为靶标,采用大孔树脂和硅胶等进行分离纯化,筛选HMG-CoA还原酶抑制剂。结果:获得4个对HMG-CoA还原酶活性具有较强抑制作用的化合物,为槲皮素、金丝桃苷、芦丁和绿原酸,4个化合物抑制率之和为50.01%,复配后的抑制活性为79.48%。结论:槲皮素和金丝桃苷为山楂中抑制HMG-CoA还原酶活性的主要活性成分,且单体之间存在协同作用。

Objective： To study the hypolipidemic active compounds from Crataegus pinnatifida and mechanism of action of those.Method： Guided by the inhibitory activity to HMG-CoA reductase,the active compounds were separated and purified with macroporous resin and silica gel.Result： Four active compounds were obtained,which were quercetin,hyperoside,rutin and chlorogenic acid,the sum of their inhibitory rate was 50.01%,and the total inhibitory rate of the mixture of four active compounds matched was 79.48%.Conclusion： Quercetin and hyperoside were the principle active components inhibiting HMG-CoA reductase in Hawthorn fruit,and there were synergistic action among them.