不同保存液保存小肠的效果评估

Evaluation of various solutions for porcine small bowel graft preservation

目的评价乳酸林格（ＬＲ）液、ＥｕｒｏＣｏｌｉｎｓ（ＥＣ）液、高渗枸椽酸盐嘌呤（ＨＣＡ）液和武汉医学院器官１号（ＭＷＯ１）液保存猪小肠的效果。方法根据不同保存液将动物随机分成４组，每组又根据保存时间不同分成３个亚组，供肠保存后行自体移植。结果小肠保存１０小时后移植，术后３周各组受体的存活率均为１００％；保存１８小时，ＬＲ液组、ＥＣ液组、ＨＣＡ液组和ＷＭＯ１液组受体的存活率分别为６０％、１００％、８０％和１００％；保存２４小时后，ＬＲ液组的移植小肠不能存活，ＨＣＡ液组、ＥＣ液组和ＷＭＯ１液组受体的存活率分别为２０％、８０％和８０％，ＥＣ液和ＷＭＯ１液保存的小肠组织损伤较轻，肠粘膜的ＡＴＰ含量及Ｎａ＋－Ｋ＋ＡＴＰ酶的活性高于ＬＲ液和ＨＣＡ液保存组。结论研究表明保存猪小肠ＥＣ液和ＷＭＯ１液优于ＬＲ液和ＨＣＡ液，前者可以有效地保存猪小肠１８小时。

Objective To study the effects of four solutions, lactate Ringer (LR) solution, Euro Collins (EC) solution, hyperosmolar citrate adenine (HC A) solution, and WMO 1 solution, on porcine small bowel graft preservation.Methods The animals were given various solutions and randomly divided into 4 groups. And each group was redivided into three subgroups based on preservation time (10 h, 18 h, and 24 h). After preservation, the porcine segmental small bowel autotransplantation was performed.Results After the small bowel preservation for 10 h, on postoperative day 21 the recipient survival rate was 100% in all groups. After the small bowel preservation for 18 h, the recipient rate was 60% (LR), 100% (EC), 80% (HC A), and 100% (WMO 1). After the small bowel preservaton for 24 h, no animals were survived more than 21 days in LR group, in HC A, EC and WMO 1 groups the recipient survival rate was 20%, 80%, and 80%, respectively. Intestinal mucosal ATP level and Na +-K +ATPase activity were higher in EC and WMO 1 groups than in LR and HC 1 groups.Conclusion Small bowel grafts could be effectively preserved for 10 h by the LR and HC A, and for 18 h by EC and WMO 1 solutions.