浙江地区炎症性肠病136例危险因素及其基因多态性分析

Epidemiological investigation on the risk factors and analysis on gene polymorphisms of inflammatory bowel disease in Zhejiang population

目的研究浙江地区住院治疗的炎症性肠病(IBD)患者的可能危险因素及NOD2/CARD15基因3个单核苷酸多态性(SNPs)与IBD的相关性。方法通过问卷调查对2005年1月至2008年12月浙江大学医学院附属第一医院、浙江省金华市中心医院、宁波李惠利医院及台州地区医院136例IBD患者和等量健康对照者进行病例对照研究,对结果进行COX回归分析;采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)法对60例无血缘关系的IBD患者及60例健康对照者NOD2/CARD15基因Arg702Trp、Gly908Arg和Leu1007fsinsC多态性进行分析。结果 IBD平均发病年龄42.6岁,男性稍多于女性。牛奶、油炸食品和精神因素在IBD致病因素中具有统计学意义。IBD患者和健康对照者均未检测出纯合子和杂合子的单核苷酸多态性(SNPs)突变。结论牛奶、油炸食品和精神紧张可能是浙江地区IBD潜在的危险因素,白种人克罗恩病(CD)患者相关易感基因NOD2/CARD15常见的3个SNPs与浙江地区IBD患者无关。

Objective To screen the risk factors by case investigation and determine three single nucleotide polymorphisms （SNPs） of NOD2/CARD15 gene in inflammatory bowel disease （IBD） of Zhejiang population. Methods 136 determined IBD patients and 136 paired healthy controls were surveyed with an organized inventory comprising of relevant items to IBD. COX regression method was used to screen the statistically significant risk factors for IBD. The poiymorphisms of NOD2/CARD15 gene Arg702Trp, Glyg08 Arg and Leul007fsinsC were gen- otyped in 60 patients with IBD and 60 healthy controls by polymerase chain reaction and restriction fragment length polymorphism. Results IBD occurred primarily in young adults with a mean age of 42. 6 years. The ratio of males to females was 1.23： 1. COX regression indicated the statistical significance in milk,fried food and stress over the other postulated risk factors for IBD. None of the patients with IBD and healthy controls had heterozygous or homozygous SNPs variants. Conclusion Milk,fried food and stress are the potential risk factors for IBD. The common variants in NOD2/CARD15 gene found in Caucasians with Crohn disease are not associated with IBD in Zhejiang population.