非小细胞肺癌MEST基因的表达及印迹状态

Genomic imprinting of MEST in nonsmall-cell lung cancer

目的探讨中胚叶特异性转录子(又名父源表达基因-1)(MEST)的基因印迹在非小细胞肺癌发生发展中的作用。方法利用聚合酶链反应(PCR)技术结合限制性片段长度多态性(PFLP)技术,分析32例非小细胞肺癌及其对应的癌周正常肺组织中MEST基因的表达及其印迹状态。结果 11例MEST杂合子信息样本中,9例(81.8%)肺癌组织发生了印记缺失(LOI),其中6例为低等级、低发展阶段的肿瘤,而与之对应的癌周组织除1例为弱的双等位基因表达之外,均为单等位基因表达。同时11份MEST杂合子信息样本肺癌组织中MEST的平均表达水平是癌旁组织的1.5倍,且二者表达量差异有显著性(P<0.01)。结论 MEST基因的印迹缺失参与了非小细胞肺癌的发生发展过程。

Objective To study the imprinting status and expression level of Mesoderm-specific transcript/ Paternally expressed gene 1 （MEST） in the development of nonsmall-cell lung cancer （ NSCLC ）. Methods The imprinting status and expression level of MEST was studied among 32 NSCLC patients by using PCR and RFLP. Results 11 of 32 patients（34.4% ） studied were informative for MEST. LOI of MEST was noted in 81.8% （9 of 11 ） of informative cases, among which 6 cases were low grade, undeveloped tumors. Monoallelic expression （ except one case ） was maintained in the matched normal tissues examined. Conclusion The findings indicated that LOI of MEST was involved in the development of NSCLC. Meanwhile, 11 informative samples of MEST, the average expression of MEST in lung cancer tissue was 1.5 times of the MEST in adjacent tissue, with significant difference.