猪圆环病毒1型天津株全基因组的克隆与序列分析

Cloning and Sequence Analysis of the Complete Genome of Porcine Circovirus Type 1 Tianjin Strain

从天津地区分离和克隆出1株猪圆环病毒1型(PCV1),并进行基因组序列分析。参照GenBank上PCV1全基因组序列设计1对引物,用PCR技术从天津一猪场的临床发病猪病变组织中扩增和克隆获得1株PCV1全基因组序列,并进行序列测定和分析。全基因组序列结果显示,该株PCV1基因组全长1759bp,与国内外其他各株PCV1的核苷酸同源性达98.2%以上。主要开放阅读框(ORF)序列结果显示,该分离株与国内外其他PCV1毒株间的ORF1与ORF2的核苷酸同源性分别为97.5%～99.8%和92.7%～99.9%。虽然各个PCV1分离株之间的全基因组与主要ORF的核苷酸同源性均很高,但还是呈现出一定的地域相关性。

To isolate and clone one porcine circovirus type 1 （PCV1） strain from Tianjin, and analyze its genome sequence, a pair of primers for porcine circovirus type 1 （PCV1） was designed according to its sequences available in GenBank, and the genome sequence of one PCV1 isolate was amplified by PCR assay with the tissues of the clinical diseased from a pig farm in Tianjin. Then, the complete genome of the PCV1 isolate was cloned, sequenced and analyzed. The genome sequence results showed that this genome is 1759 nt in size, and shared more than 98.2% identity of nucleotide with other PCV1 isolates selected from the GenBank. The sequences of the main open reading frame （ORF） revealed that there were about 97.5%-99.8% and 92.7%-99.9% identity of nucleotide in the ORF1 and ORF2 among the selected PCV1 isolates respectively. The present study indicated that there was a geographical correlation among the PCV1 isolates, although the nucleotide identity of the genomes and main ORFs of the selected PCV1 isolates was relatively high.