山东近海牙鲆同工酶的生化遗传分析

BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.ET S.) IN THE COASTAL WATERS OF SHANDONG

1996年5月，1997年1月、12月和1998年4月在山东青岛和荣成近海采集了131尾活牙鲆共710个生化样品，采用水平淀粉胶和垂直聚丙烯酰胺凝胶电泳的方法，对牙鲆的25种同工酶进行了分析。结果检测出了LDH、MDH、MEP、IDHP、G3PDH、AK、CK、ACP、PGM、SDH、ADH、CATSOD、GDH和GPI等15种同工酶在牙鲆眼睛、肌肉、心脏、肝脏、胸鳍和肾脏等6种组织或器官中的表达情况。对牙鲆15种同工酶进行了生化遗传分析，获得了基本酶谱。比种同工酶共记录出29个基因座位，其中9个基因座位Ldh-A、Ldh-C、Idhp-1、Acp-1、Pgm、Sdh、Adh、Cat和Gdh为多态，其多态座位比例为31．03％。

131 live Ieft-eyed flounders, Paralichlhys olivaceus (T. et S.), were collected from the coastri waters of Qngclao and a fishing farm in Rongcheng City, Shandong Province from May in 1996 to April in 1998. In total, 710 biochemical samples from 6 tissues and organs (eye, muscle, heart, liver, pectoral fin and kidney) were fetched and stored immediately in liquid nitrogen (-196 ℃). The supematant of samples to be used for the experiments were extracted by homogenizing and centrifuging. 15 isozymes (LDH, MDH, MEP, IDHP, G3PDH, AK, CK, ACP, PGM, SDH, ADH, CAT, SOD, GDR GPI) in 3 buffer systems (TC, pH = 6.9; EBT, pH = 8.9; TG pH = 8.3) were tested by horizontal sath gel and vertical POlyacrylthede ge1 electroporesis, and their expression in 6 tissues and organs-tissues specify was also tested. The biochendcal genetics were analyzed in terms of their locus number, structure, alleles, etc., on the basis of which the basic electrophoretic patterns of these l5 isozymes were gained. The electrophoretic patterns of the rest isozymes were not recorded. Altogether 29 gene loci were recorded, among which 9 gene loci including Ldh-A (alleles, 100, 167), Ldh-C (alleles, 98, 100), Idhp-1 (alleles, 78, 100), Acp-1 (alleles, 78, 100), Pgm (alleles, 100, 200), an (alleles, 100, 125), Adh (alleles, 70, 100, 150), Cal (alleles, 93, 100 , 140) and Gdh (alleles, 100, 130) are Polymorph, and the mean proportion of polymorph loci was 31.03%. In the meanhme, the expression of ldh-A and Ldh-B during the biochemical genetic analysis of LDH on Paralichlhys olivaceus (T. et S.), the interrelationships between the activities of isozymes protein and stored condition of samples, and testing of effective genetic markers were also discussed.