食管癌组织中血管生成素样蛋白3及MVD的表达

Expression of ANGPTL3 and microvessel density in esophageal carcinoma

目的:研究食管癌组织中血管生成素样蛋白3(ANGPTL3)的表达情况及其与血管生成的关系。方法:应用免疫组化SP法检测52例食管癌组织(TNMⅠ期21例,Ⅱ期17例,Ⅲ期14例)和相应正常食管组织中AN-GPTL3的表达,同时检测了微血管密度(MVD)。结果:正常食管组织和食管癌组织中ANGPTL3蛋白的阳性表达率差异有统计学意义(χ2=31.243,P<0.001)。Ⅲ期食管癌组织中ANGPTL3的阳性表达率(92.9%,13/14)高于Ⅰ期(57.1%,12/21)、Ⅱ期(82.4%,14/17)患者,差异有统计学意义(χ2=6.443,P=0.040)。食管癌组织中MVD为47.2±6.7。随着肿瘤期别的增加,MVD>47.2的例数也随之增多(χ2=7.648,P=0.022)。食管癌组织中AN-GPTL3蛋白表达与MVD(MVD>47.2)呈正关联关系(rP=0.484,P<0.001)。结论:食管癌组织中ANGPTL3高表达是肿瘤血管生成的重要因素。

Aim：To investigate the expression of angiopoientin-like protein 3（ANGPTL3）and its relationship with the angiogenesis in esophageal carcinoma.Methods：The expression of ANGPTL3 and the microvessel density（MVD） in 52 specimens of esophageal carcinoma（TNM stages：Ⅰ 21 cases,Ⅱ 17 cases,Ⅲ 14 cases） and corresponding normal esophageal tissues were detected by SP immunohistochemistry.Results：Significant difference expression of ANGPTL3 was noted between the groups of normal esophageal tissue and esophageal carcinoma tissue（χ2=31.243,P0.001）.The positive rate of ANGPTL3 in Ⅲ stage（92.9%,13/14）was obviously higher than that inⅠstage（57.1%,12/21）and Ⅱstage（82.4%,14/17）.The difference was statistically significant（χ2=6.443,P=0.040）.The MVD was 47.2±6.7 in esophageal carcinoma tissue,and the cases with MVD47.2 was more with advance of TNM stages（χ2=7.648,P=0.022）.There were positively relationship between the expression of ANGPTL3 and MVD in esophageal carcinoma（rP=0.484,P0.001）.Conclusion：High expression of ANGPTL3 may play an important role in tumor angiogenesis of esophageal carcinoma.