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藏獒冻精畸形率检查中染色方法的比较 被引量:2

Comparison on Staining Methods Used for Detection of Sperm Deformity Rate of Tibetan Mastiff
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摘要 分别采用Giemsa染色法、曙红染色法对藏獒冷冻精液涂片染色,以检查精子畸形率。结果表明,曙红染色法对藏獒精子头部染色适中,头部轮廓清晰,顶脊清楚,顶体明显,中段和尾部畸形检查清晰;两种染色方法检测的精子畸形率比较,差异不显著(P>0.05)。由试验可知,曙红染色液配制简便,可作为检测藏獒精子畸形与否的优选方法。 Frozen sperm of Tibetan mastiff were smeared and stained by Giemsa stain and eosin to observe sperm deformity.The results showed that the heads of sperm stained by eosin were stained moderately,and the sperm had a clear head,clear ridge and acrosome,and clear deformity of middle and tail part.Sperm deformity rates were not significantly different between the two staining methods.Because eosin stain can be prepared simply,eosin staining can be used as the optimal method to detect sperm deformity.
作者 胡宁玺
出处 《畜牧与饲料科学》 2010年第8期29-30,共2页 Animal Husbandry and Feed Science
关键词 藏獒精子 畸形率 染色法 Tibetan mastiff sperm deformity rate staining
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