酶电极流动注射分析法测定葡萄糖

Determination of Glucose by An Enzyme Electrode Flow Injection Analyze System (EFIA)

一种酶电极流动注射分析系统(EFIA)用于血糖和发酵葡萄糖的快速测定。研究了酶电极及其工作系统的性能和各种影响参数,,奠定了实用化基础。

An enzyme (glucose oxidase, GOD) electrode flow injection system(EFIA) has been developed for clinical determination of glucose in blood.The study focused on the factors affecting the sensor response. The linear range of the sensor shifted from 0-200 mg/dL to 0-2000 mg/dL by different injection volumes, the linear range increased with the loss of sensitivity .Temperature coeffecient (Q10) was 1.12. The optimal pH value for the sensor activity was 5.8-6.5. The electrode response was evidently depressed by the lower oxygen concentration in sample solution, however, no influence was observed as the oxygen saturation was beyond 80% with a sample size of 100μL or beyond 10% in a 10μL sample. The presence of sodium citrate (3.5%) and saline (NaCI, 0.85%) enhanced response slightly. When mixing glucose with human plasma or broth medium,approximately 99.8% or 96.2% recovery was obtained. The EFIA has been used for clinical blood sugar assay, the correlation coeffecients, r, were 0.992 between EFIA and conventional o-toluindine method and 0.982 between EFIA and Automatic Dichromatic Analyzer(ABA 100). Under working conditions (37±0.01℃,pH 6.5, 224mL/h of flow rate and 50μL of sample injection volume), time spent for one assay was about 1.5