亚硒酸钠诱发大鼠白内障晶状体前列腺素生物合成的研究

Biosynthesis of PGE_2 and PGF_2α in Normal and Sodium Sele nite Induced Cataractous Lenses

用亚硒酸钠诱发大鼠产生白内障后,将晶状体微粒体与外源性花生四烯酸共同孵育,用放射免疫方法测定白内障晶状体前列腺素E_2(PGE_2)及前列腺素F_2α(PG-F_2α)的生物合成情况,并与正常晶状体进行了比较,结果表明大鼠晶状体具有酶促合成PGs的能力。正常晶状体及白内障晶状体合成PGE_2的能力分别为687.75±113.97及1095.00±79.39pg/100mg晶状体湿重/15分钟,PGE_2α则分别为51.45±36.72及158.83±115.94pg/100mg晶状体湿重/15分钟(平均数±S.D.)。这说明大鼠白内障晶状体合成PGs的能力明显增高,与正常晶状体相比有显著性差异(PGE_2P<0.001,PGF_2αP<0.02)。在前2次注射亚硒酸钠后,大鼠白内障晶状体PGs的合成能力逐渐高于正常晶状体,并随注射亚硒酸钠的次数增加和白内障晶状体混浊程度加重,PGs在晶状体内的含量增加。

The relationship between the development of sodium selenite induced cataractogenesis in rat and biosynthesis of prostaglandins (PGs) by their lens mi-crosomes was studied. After cataract induced by injection of sodium selenite, the lens microsomes were prepared and incubated with 200μm arachidonic acid for 15 min at 37℃. Biosynthesis of PCE2 and PGF2α by normal and cataractous lens microsomes were measured by RIA. The results showed that the cataractous lenses had higher ca pabilities in the synthesis of PCE2 and PGF2α than the normal lenses. Under same experimental conditions and with the results expressed in pg per 100mg of lens wet weight in 15 min, the cataractous and normal microsomes synthesized PGE2 1095.00±79.39 and 687.75±113.79, and PGF2α 158,83±115.94 and 51.45±36.72 respectively. The difference between cataractous and normal lenses was significant, (PGE2 P<0.001, PGF2α P<0.02) and the more the injection of sodium selenite rats received, the higher the results were obtained.