冬油菜和春油菜在冷驯过程中膜脂分子的变化

Profiling the Changes of Molecular Species in Membrane Lipids during Cold-acclimation in Winter and Spring Cultivars of Rapeseed(Brassica napus)

研究了冬油菜和春油菜两种油菜品种在4℃冷驯3天后11类151种膜脂分子的变化,计算了其中膜脂的相对含量、双键指数和碳链长度值。结果发现油菜膜脂的膜脂分子组成模式及其冷驯诱导的膜脂分子组成变化与前期实验中的拟南芥情况非常相似,冷驯3天诱导的膜脂分子组成变化较小,但是膜脂的双键指数略有升高,春油菜双键指数和冷驯积累的脯氨酸含量都比冬油菜多。上述结果说明,油菜的冷驯需要更多的时间,春油菜对冷驯比较敏感。

The changes of molecular species in membrane lipids during cold-acclimation （CA） in winter and spring cultivars of rapeseed （Brassica napus） were profiled with ESI-MS/MS based lipidomics. The membrane fluidity,the critical properties for plants to tolerate freeing injuring,was examined with double bond index （DBI） and carbon number of the fatty acid of the glycrolipid. The results indicated that the molecular composition of membrane lipids in the two cultivars under both normal growth condition and CA were similar to that of Arabidopsis reported previously and that DBI in spring cultivar accumulation subtly more than that in winter cultivar. The results suggested that CA of rapeseeds needs more than 3 days and that spring cultivar were more sensitive to CA,which was confirmed by the CA-induced proline accumulation.