胰腺癌中14-3-3蛋白各亚型的表达及其临床意义

Expressions of 14-3-3 protein isoforms and their significance in pancreatic ductal adenocarcinoma

目的探讨14-3-3蛋白各亚型在人胰腺导管腺癌组织中的表达情况及其临床意义。方法采用石蜡包埋组织进行免疫组化染色,分析14-3-3蛋白各亚型在30例胰腺癌和19例胰腺癌旁正常组织中的表达状态,探讨其与胰腺癌临床病理参数之间的关系。结果 14-3-3蛋白β、γ、ε、ζ、η、θ和σ7个亚型在胰腺癌组织原发灶中表达阳性率分别为70%(21/30)、83.33%(25/30)、26.67%(8/30)、73.33%(22/30)、33.33%(10/30)、30%(9/30)和43.33%(13/30)。其中癌旁正常胰腺组织的β、γ、ζ、η和σ亚型阳性率与胰腺癌组织比较差异显著(P<0.05),ε和θ亚型阳性率比较差异不显著(P>0.05);14-3-3蛋白阳性表达率与胰腺癌的分化程度、恶性级别及淋巴结转移均无明显关系(P>0.05)。结论 14-3-3蛋白7个亚型中有5个在胰腺癌组织中高表达,表明该蛋白在胰腺癌的发生过程中具有重要作用,并且有可能成为胰腺癌基因治疗新的靶点。

Objective To investigate the expression of 14-3-3 proteins and their clinical significance in pancreatic ductal adenocarcinoma. Methods Seven 14-3-3 protein isoforms （β、γ、ε、ζ、η、θ and σ） were examined in 30 pancreatic cancer tissues and 19 adjacent normal pancreatic tissues by immunohistochemieal EnVision method. Results The positive rate of protein 14-3-3 β、γ、ε、ζ、η、θ and σ isoforms in pancreatic cancer tissues was 70. 00% （ 21/30 ）, 83.33% （25/30）, 26. 67（8/30）, 73.33% （22/30）, 33.33 （10/30）, 30. 00 （9/30） and 43.33（13/30）, and those in adjacent normal pancreatic tissues was 5.26% （ 1/19 ） , 15.79% （3/19） , 42. 11% （8/19） , 5. 26% （1/19） , 5. 26% % （1/19）, 21.05% （4/19） and 5.26% （1/19）, respectively. A significant difference was found between the two groups in the expression of β、γ、ζ、η and σ isoforms. But no relationship was found between 14-3-3 proteins overexpression and differentiation, malignant degree, or lymph node metastasis. Conclusions The expression of 14-3-3 β、γ、ζ、η and σ protein is higher in pancreatic cancer than adjacent normal pancreatic tissues. They may play an important role in the tumorigenesis of pancreatic ductal adenocareinoma.