丁香苷对大鼠佐剂性关节炎的治疗作用及其机制

Therapeutic effect of syringin on adjuvant arthritis in rats and its mechanisms

研究丁香苷对佐剂性关节炎(AA)大鼠的治疗作用及其机制。采用大鼠足跖皮内注射弗氏完全佐剂诱发大鼠AA模型。足容积法测量继发侧足肿胀度,进行疼痛评分和多发性关节炎评分,MTT法检测刀豆蛋白(ConA)和脂多糖(LPS)诱导的脾淋巴细胞增殖反应,酶联免疫吸附测定法(ELISA)测定ConA诱导的脾淋巴细胞产生IL-2的水平以及脂多糖(LPS)诱导的腹腔巨噬细胞(PMΦ)产生IL-1β和TNF-α的水平。结果表明,大鼠致炎后第14天,继发性关节炎出现,同时灌胃给予不同剂量的丁香苷及阳性对照药雷公藤多苷,连续给药16天。丁香苷各剂量组于第22天开始不同程度降低AA大鼠继发侧关节肿胀度、关节疼痛评分、多发性关节炎指数;同时各剂量组可不同程度地提高AA大鼠低下的脾淋巴细胞增殖反应和脾细胞IL-2的产生,降低AA大鼠PMΦ产生过高的IL-1β和TNF-α。这些结果提示,丁香苷可以改善AA大鼠异常的细胞免疫功能并调节细胞因子的平衡,可能是其治疗AA大鼠继发性炎症的作用机制。

This study is to investigate the therapeutic effect of syringin on adjuvant arthritis （AA） in rats and its mechanisms. Complete Freund＇s adjuvant （FCA） was used to induce AA in rats. Secondary paw swelling of AA rats was measured with volume meter. Pain response and polyarthritis index were scored. Meanwhile,splenic lymphocyte proliferation response induced by concanavalin A （ConA） or lipopolysaccharide （LPS） was examined with MTT assay. IL-2 production of splenic lymphocytes and IL-1β,TNF-α production of peritoneal macrophage （PMΦ） were estimated by enzyme linked immunosorbent assay （ELISA）. The secondary inflammation of AA rats appeared on the 14th day after injection of FCA. Syringin and tripterygium glycosides （TG） were given by intragastric administration for 16 days from the 14th day. Treatment of AA rats with syringin and TG from the 22th day significantly attenuated the secondary hind paw swelling,as well as relieved the pain response and the polyarthritic symptoms of the whole body as compared with that of the AA model group. The suppressed lymphocyte proliferation and IL-2 production of splenic lymphocytes in AA rats were reversed by treatment with syringin. Meanwhile,syringin remarkably down-regulated IL-1β,TNF-α productions from PMΦ. These results indicate that anti-inflammatory effects of syringin on AA rats are mediated by modulating the immune function of abnormal cells and the balance of cytokines.