液相色谱-电喷雾串联质谱法分析新乌头碱在大鼠尿液中的代谢产物

Analysis on the metabolites of mesaconitine in the rat urine by liquid chromatography and electrospray ionization mass spectrometry

研究新乌头碱在大鼠尿液中的主要代谢产物。大鼠灌胃给予新乌头碱,连续收集尿液24h,尿液经固相萃取法富集纯化后,以液相色谱-电喷雾多级串联质谱法分析尿液中新乌头碱的代谢产物。在大鼠尿液中发现了新乌头碱原形药物和5个代谢产物,分别推测为新乌头次碱葡糖醛酸结合物、10-羟基新乌头碱、1-O-去甲基新乌头碱、去氧新乌头碱、新乌头次碱。在新乌头碱的5个代谢产物中,新乌头次碱葡糖醛酸结合物(m/z766)为首次发现新的新乌头碱在大鼠体内的II相代谢产物,揭示了新乌头碱在大鼠体内代谢新途径。

The mesaconitine and its major metabolites in the rat urine were identified by liquid chromatography and electrospray ionization tandem mass spectrometry. The rat urine was collected for consecutive 24 hours from the rat following intragastric infusion of mesaconitine,subsequently which were enriched and purified using solid phase extraction. The metabolites of mesaconitine in the rat urine were analyzed by the liquid chromatography and electrospray ionization tandem mass spectrometry. It is shown that the parent drug mesaconitine and its metabolites were found in the rat urine,such as hypo-mesaconitine glucuronic acid conjugate,10-hydroxy-mesaconitine,1-O-demethyl mesaconitine,deoxy-mesaconitine and hypo-mesaconitine. Among the five of metabolites,the hypo-mesaconitine glucuronic acid conjugate （m/z 766） was first discovered as the aconitine in rats phase Ⅱ metabolites,which revealed a new way of mesaconitine metabolism in rats.