2-氨基-4,5-二芳基嘧啶的合成及对PC12细胞缺氧缺糖的保护作用

Synthesis of 2-amino-4, 5-diarylpyrimidines and their protective effects against oxygen-glucose deprivation in PC12 cells

以异黄酮和盐酸胍为原料,合成了6种未见报道的2-氨基-4,5-二芳基嘧啶类化合物,采用IR、1HNMR、13CNMR和元素分析对其结构进行了表征。采用MTT法,研究了6种2-氨基-4,5-二芳基嘧啶类化合物对连二亚硫酸钠导致PC12细胞缺氧缺糖的影响。实验表明,6种2-氨基-4,5-二芳基嘧啶对PC12细胞缺氧缺糖损伤均有神经保护作用,可提高损伤模型中PC12细胞的存活数,降低细胞损伤程度。

A series of 6 novel 2-amino-4, 5-diarylpyrimidines were synthesized by the reaction of isoflavones with guanidine. They were identified by the spectra of IR, 1H NMR, 13C NMR and elemental analysis. The protective effects of 2-amino-4, 5-diarylpyrimidines against oxygen-glucose deprivation leading by sodium hydrosulfite in PC12 cells were studied by MTT method. It was shown that 2-amino-4, 5-diarylpyrimidines exhibited neuroprotective effects against oxygen-glucose deprivation injury in PC12 cells, which could increase the amount of survival PC12 cells and reduce their injured degree in the model.