盆腔脏器脱垂患者阴道壁组织MMP-1、TIMP-1和Collagen I表达的研究

The Expression of MMP-1,TIMP-1 and Collagen Ⅰ in the Vaginal Tissue of Patients with Pelvic Organ Prolapse

目的:探讨盆腔脏器脱垂(POP)患者盆底支持结构胶原代谢与疾病发生发展的关系。方法:选择无压力性尿失禁(SUI)POP手术治疗患者30例为POP组,以同期无SUI和POP,因子宫良性病变行阴式全子宫切除术患者30例为对照组。采用免疫组化三步法检测阴道壁组织基质金属蛋白酶-1(MMP-1)、组织金属蛋白酶抑制剂-1(TIMP-1)和Ⅰ型胶原(CollagenⅠ)的表达情况。结果:POP组阴道前壁组织中MMP-1表达显著高于对照组(P<0.05),TIMP-1的表达显著低于对照组(P<0.05),CollagenⅠ的表达显著低于对照组(P<0.05)。3者之间存在相关关系,其中CollagenⅠ与MMP-1呈负相关(r=-0.961,P<0.05),CollagenⅠ与TIMP-1呈正相关(r=0.982,P<0.05),MMP-1与TIMP-1呈负相关(r=-0.977,P<0.05)。结论:POP患者阴道壁组织中MMP-1表达增强而其抑制剂TIMP-1表达降低,导致CollagenⅠ分解增加可能是POP的发病机制之一。

Objective： To investigate the relation of collagen metabolism in patients with pelvic organ prolapse （POP） and the progression of POP. Methods：Biopsy specimens of vaginal wall tissue from 30 women with POP and another 30 patients without POP （as control group）were collected. The expression of MMP-1, TIMP-1 and Collagen I were checked by immunohistochenical technique. Results： Expression of MMP-1 in POP group was significantly higher than that in control group （ P 〈 0.05）, while the expressions of TIMP-1 and Collagen I in POP group were significantly lower than those in control group（ P 〈 0.05）. A correlation was found among the three parameters. Collagen I was negatively correlated with MMP-1 （r =-0. 961, P 〈 0.05）, and positively correlated with TIMP-1 （ r = 0. 982, P 〈 0.05）. Also, MMP-1 was negatively correlated with TIMP-1 （ r =-0.977, P 〈 0.05 ）. Conclusions： The expression of MMP-1 is strengthened and the expression of TIMP-1 is decreased in the vaginal tissue of POP patients, so the sequential breakdown of Collagen I might be the pathogenesis of POP.