有氧运动对ApoE基因缺陷小鼠脂肪组织脂联素mRNA表达的影响

Effect of Aerobic Exercise on the Expression of Adiponectin mRNA in Mice with ApoE-Deficiency

目的:探讨运动对ApoE基因缺陷小鼠脂肪组织脂联素mRNA表达及其防治动脉粥样硬化(AS)的作用机理。方法:20只8周龄雄性ApoE基因缺陷小鼠随机分为安静组(C,10只)和运动组(E,10只)。两组小鼠均饲以高脂膳食饲料,C组不训练,E组进行14周跑台运动,前6周由10m/min×30min逐渐递增至13m/min×60min,后8周稳定在13m/min×60min,跑台坡度为0,每周5次。14周末取材比较两组小鼠体重、内脏脂肪重量及脂体比,观察两组小鼠主动脉病理变化,检测比较其脂肪组织脂联素mRNA表达水平、血清脂联素水平及血脂水平。结果:与C组相比,E组小鼠AS斑块着色区域较小;E组小鼠脂肪组织脂联素mRNA表达水平显著高于C组(P<0.05),其血清脂联素含量也显著高于C组(P<0.01)。两组小鼠血清TC、TG、LDL、HDL水平均无显著性差异。结论:14周有氧跑台运动显著提高了小鼠脂肪组织脂联素mRNA表达及血清脂联素水平,这可能是有氧运动防治AS的重要机制之一。

Purpose T he purpose of present study is to observe the effect of aerobic exercise on the adiponectin（APN） mRNA and the mechanism of the effect in preventing arteriosclerosis. Methods Twenty 8-week-old mice with ApoE deficient were randomly assigned to two groups： control group（C）and aerobic exercise group（E）. The mice were fed with high fat diet. Mice in group E ran on a treadmill with the load from 10m/min×30min up to 13m/min×60min, 5 days/week, for a total of 14 weeks. At the end of experiment, all mice were killed, and the blood, and epididymal and perirenal fat were obtained. Atherosclerotic change was observed. APN mRNA in adipose tissue was tested by RT-PCR. Serum APN and lipid levels were tested by ELISA. Results It was shown that the area of atherosclerotic plaque in group E was significantly less than in group C; APN mRNA expression in the adipose tissue and serum APN level in group E were significantly higher than that in group C（P0.05）;however,the serum TC,TG,LDL,HDL levels were not significantly different between two groups. Conclusion It was suggested that 14-week aerobic exercise significantly increased serum APN level and APN-mRNA in the adipose tissue. Aerobic exercise seems to be one of the factors for preventing atherogenesis.