人骨髓源和胎盘源间充质干细胞对T细胞增殖抑制作用的比较

Comparison the inhibitory effects of human bone marrow mesenchymal stem cells and human palacenta mesenchymal stem cells on T cell proliferation

目的:比较研究人骨髓源和胎盘源间充质干细胞介导的T细胞增殖抑制作用机制。方法:应用流式细胞术(FCM)分别检测B7H4和PDL1在人骨髓源间充质干细胞(HBMSCs)和胎盘源间充质干细胞(HPMSCs)上的表达;应用抗体阻断试验分析B7H4、PDL1分别在HBMSCs和HPMSCs对T细胞增殖及周期影响中的作用。结果:HBMSCs上高表达免疫负性调控分子B7H4,而HPMSCs上高表达免疫负性调控分子PDL1。分别应用B7H4mAb和PDL1mAb阻断,可使HBMSCs和HPMSCs对PHA激发的T细胞增殖抑制作用明显减弱;下调T细胞周期中G0/G1期细胞数量,上调S期细胞数量,明显减弱HBMSCs和HPMSCs对T细胞周期的影响。结论:HBMSCs和HPMSCs可通过表达不同的免疫负性调控分子介导T细胞的增殖抑制作用。

AIM： To compare and study the inhibitory effects of human bone marrow mesenchymal stem cells（HBMSCs） and human palacenta mesenchymal stem cells（HPMSCs） on T cell proliferation,and the underlying mechanism.METHODS： The expression of B7H4 on HBMSCs or the expression of PDL1 on HPMSCs were detected by FCM.Blocking experiment was used to analyze the effects of B7H4 or PDL1 on HBMSCs or HPMSCs mediating suppression on T cell proliferation and cell cycle.RESULTS： FCM detection showed that HBMSCs highly expressed B7H4,while HPMSCs highly expressed PDL1,the negative immune molecules.Blockade B7H4 on HBMSCs with B7H4mAb significantly attenuated the inhibitory effects of HBMSCs on T cell proliferation.Likewise,blocking the expression of PDL1 on HPMSCs obviously weakened the suppressive effects of HPMSCs on T cell proliferation activated by PHA.Moreover,Blockade B7H4 on HBMSCs with B7H4mAb or PDL1 on HPMSCs with PDL1mAb significantly weakened the inhibitory effects of HBMSCs or HPMSCs on T cell cycle through down-regulating the cell number in G0/G1 phase and up-regulating the cell number in S phase.CONCLUSION： HBMSCs and HPMSCs could mediate the suppressive effects on T cell proliferation through expressing different negative immune molecules.