葱白提取物对创伤性兔下肢深静脉血栓形成过程中血小板活化的影响

Effect of Fistular Onion Stalk Extracts on Platelet Activation during the Process of Post-traumatic Deep Vein Thrombosis in Rabbits

目的:通过检测兔创伤性下肢深静脉血栓形成P-选择素(CD62P)、血小板活化因子(PAF)及溶酶体膜蛋白(CD63)的表达,探讨葱白提取物防治深静脉血栓的疗效和机制。方法::随机将新西兰白兔24只分为空白模型组、葱白防治组和葱白治疗组。按照赵学凌方法构建兔下肢创伤性深静脉血栓模型,造模成功后,空白模型组给予生理盐水,葱白治疗组给葱白提取物1周,葱白防治组造模前持续给葱白提取物1周,造模后同葱白治疗组。并检测造模前后CD62P、PAF和CD63的变化,对实验数据进行统计分析。结果:造模后,各组兔血清中CD62P、PAF、CD63含量明显升高(P<0.05),葱白防治组上升幅度较小(P<0.05),空白模型组和葱白治疗组上升幅度较大(P<0.01),葱白提取物预防和治疗均可以降低模型组兔血清中CD62P、PAF、CD63含量(P<0.05),和葱白治疗组比,葱白防治组降低CD62P和CD63水平较明显,差异有统计学意义(P<0.05),而对PAF的影响比较差异无统计学意义(P>0.05)。同时葱白提取物预防给药可以降低正常兔血液中CD62P的水平。结论:葱白提取物能防治创伤后深静脉血栓的形成,其机制与减少血小板活化,抗氧化损伤,减少血小板聚集有关。

Objective：To explore the curative effect and the possible mechanisms of fistular onion stalk extract（FOSE） on prevention and treatment of deep vein thrombosis（DVT） via measuring the levels of CD62P,platelet activating factor（PAF）,and CD63.Methods：New Zealand white rabbits were randomized into control group,FOSE prevention and treatment group,and FOSE treatment group.DVT models were constructed according to Zhao Xueling＇s method.Control group were given saline,FOSE prevention and treatment group were given FOSE consecutively for a week before modeling and FOSE treatment group were given onion extract consecutively for a week after modeling.The levels of CD62P,PAF and CD63 were measured and analyzed statistically.Results：The serum levels of CD62P,PAF and CD63 were significantly increased（P0.05） after modeling.Such change was higher in FOSE prevention and treatment group than in control group and FOSE treatment group（P0.05）.Both prevention and treatment of FOSE decreased the levels of serum CD2P,PAF and CD63.Compared with FOSE treatment group,the levels of CD62P and CD63 were decreased in FOSE prevention and treatment group.There was no significant difference in PAF between the two groups.FOSE reduced the level of CD62P in normal rabbits.Conclusion：FOSE can prevent and cure post-traumatic DVT with the mechanisms involving the reduced platelet activation,oxidative damage and platelet aggregation.