原发性肝细胞癌组织免疫微环境的分析

Investigation of immune microenvironment of primary hepatocellular carcinoma

目的:探讨原发性肝细胞癌(hepatocellular carcinoma,HCC)组织中免疫细胞的浸润及分布情况。方法:采用免疫组化S-P法,检测采集自福建省肿瘤医院30例HCC癌组织、癌周组织(距肿瘤边缘<2cm)、非癌肝组织(距肿瘤边缘>5cm)石蜡标本中CD3、CD4、CD8、FoxP3、CD20、CD56、CD68的表达。结果:免疫组织化学结果显示,HCC患者CD3+、CD4+、FoxP3+细胞数在癌周组织中最高,癌组织中其次,非癌肝组织中最低(P<0.05);CD8+、CD56+、CD68+细胞数在癌周组织中最高,非癌肝组织其次,癌组织中最低(P<0.05);CD20+细胞数在肝癌组织、癌周组织、非癌肝组织中无明显差异(P>0.05)。肝癌组织中CD3+、CD4+、CD8+、CD56+、CD68+细胞主要分布于癌巢中的间质区,FoxP3+细胞呈散在分布。结论:肝癌组织局部微环境中,杀伤性免疫细胞减少,抑制性免疫细胞增加,从而导致局部免疫抑制状态。

Objective：To investigate the infiltration and distribution of immunocytes in primary hepatocellular carcinoma（HCC）tissues.Methods：Thirty HCC tissues were obtained from Tumor Hospital of Fujian Province.The expressions of CD3^＋,CD4^＋,CD8^＋,FoxP3^＋,CD20＋,CD56^＋,and CD68^＋ cells in liver tumor tissues,liver peri-tumor tissues and non-tumor liver tissues were detected by immunohistochemistry S-P assay.Results：Immunohistochemistry results showed that the numbers of CD3^＋,CD4^＋,FoxP3^＋ cells were highest in liver peri-tumor tissues,less in liver tumor tissues,and least in the corresponding non-tumor liver tissues（P〈0.05）;the numbers of CD8^＋,CD56^＋,CD68^＋ cells were highest in the liver peri-tumor tissues,less in non-tumor liver tissues,and least in liver tumor tissues（P〈0.05）;and the CD20＋ cell numbers had no significant difference between liver tumor tissues,liver peri-tumor tissues,and non-tumor liver tissues（P〉0.05）.CD3^＋,CD4^＋,CD8^＋,CD56^＋,and CD68^＋ cells were mainly distributed in the mesenchyma of the liver tumor tissue,and FoxP3^＋ cells were scattered among the whole tumor tissue.Conclusion：Cytotoxic immunocyte is decreased and the inhibitory immunocyte is increased in the microenvironment of hepatocellular carcinoma,which results in an immunosuppression status in tumor tissues.