摘要
目的观察熄风止颤合剂对蛋白酶抑制因子（PSI）诱导的帕金森病慢性动物模型大鼠的治疗作用，探讨熄风止颤合剂对帕金森病治疗的细胞凋亡机制。方法雄性sD大鼠背部皮下注射PSI，制备慢性帕金森病动物模型，将造模成功大鼠随机分为3组：分别以生理盐水、美多巴及熄风止颤合剂进行灌胃治疗，观察其行为变化，并分别通过Tunel法、免疫组织化学的方法观察大鼠脑多巴胺神经元的变化。结果中药熄风止颤合剂可以改善帕金森病的症状，且中药组大鼠大脑中多巴胺神经元的密度高于美多巴组，细胞凋亡指数低于美多巴组。结论熄风止颤合剂对帕金森病有治疗作用，其作用可能是通过降低多巴胺神经元的凋亡。
Objective To observe the therapeutic effect of Xifengzhichan（xfzch） mixture for rats model with chronic Parkin＇s disease induced by PSI,and investigate the apoptosis mechanism of therapeutic effect. Methods Male Sprague-Dawley rats were used to establish the model of Parkinson＇s disease by hypodermic injection of PSI on their back. The model rats were randomly divided into three groups and were administrated saline, madopar and the traditional Chinese medicine by gavage respectively. The change of behavior was observed. To investigate the change of dopaminergic neuron of rats brain, tunnel and immunohistochemistry were used. Results Xfzhch mixture can improve the behavior of Parkin, s disease and the density of dopaminergie nueron in traditional Chinese medicine group was higher than madopar group but apoptosis index is lower. Conclusion Xfzhch mixture is effective for Parkin＇s disease and maybe it reduce the apoptosis of madopar neuron.
出处
《潍坊医学院学报》
2010年第3期189-191,共3页
Acta Academiae Medicinae Weifang
基金
山东省中医药科技发展计划资助项目（2007-154）
