摘要
采用超高压高效液相色谱法(HPLC),在最优化条件下,以ACQUITY UPLC BEH C18为色谱柱、甲醇和三氟乙酸为流动相进行等梯度洗脱,可实现对预混合饲料中D-泛酸钙和D-生物素的联检。上述各组分在4 min内完全分离;各组分浓度与峰面积在5～200 mg/mL呈良好线性关系,检测下限(S/N=3)分别为1.5μg/mL和1.5μg/mL。
Using ultrahigh-performance liquid chromatography（UPLC） could jointly determine D- calcium pantothenate and D-biotin levels in premixed feeds with an ACQUITY UPLC BEH C18 as a chromatographic column, methanol and trifluoroacetic acid as a mobile phase, and constant gradient elution. The above two components were completely separated within 4 minutes, and each component＇s concentration presented a good linear relationship with the peak area in the range of 5-200 mg/mL. The detection limit（S/N = 3） was 1.5 μg/mL for D-calcium pantothenate and 1.5μg/mL for D-biotin.
出处
《上海农业学报》
CSCD
北大核心
2010年第3期124-126,共3页
Acta Agriculturae Shanghai
