实验动物CAR杆菌16SrRNA基因PCR监测方法的建立

Establishment of a PCR Assay for Detection of Cilia-Associated Respiratory Bacillus in Laboratory Animals Based on 16SrRNA Gene Fragment Sequence

目的建立CAR杆菌的PCR监测方法 ,筛查国内部分实验动物样本中CAR杆菌携带状况。方法利用CAR杆菌的特有16SrRNA基因序列片段267bp设计引物,通过从日本实验动物中央研究所获取的CAR标准株DNA,建立实验动物CAR杆菌16SrRNA基因PCR监测方法。结果利用建立的CAR杆菌16SrRNA基因PCR监测方法对国内455份实验动物样本进行筛查,未检出CAR杆菌感染。结论建立了敏感性好,特异性高的实验动物CAR杆菌PCR监测方法 ,未见动物携带CAR杆菌。

Objective To establish a PCR assay for detection of cilia-associated respiratory bacillus,and to identify the infectious status of clinical samples from mice,rats,mini pigs,rhesus monkeys,etc. Methods The specific primers were designed according to the 267 bp 16SrRNA gene fragment sequence. Then the PCR assay was established by using DNA copy from Japan. Results Four hundred fifty five clinical samples were tested by this established method and no positive CAR bacillus infection was detected. Conclusion The method established in this study is efficient with high sensitivity and specificity. The infection of CAR bacillus hasn’t been detected in laboratory animals in Beijing up to now.