摘要
目的:探讨STAT3 mRNA、EGF mRNA和EGFR mRNA在口腔鳞状细胞癌组织中的表达及其临床意义。方法:采用原位杂交法检测60例口腔鳞癌患者的癌组织、30例不典型增生组织及60例正常口腔黏膜组织中STAT3 mRNA、EGF mRNA和EGFRmRNA的表达,并结合病理分级、有无淋巴结转移等临床指标进行统计学分析。结果:在口腔鳞癌癌变过程中STAT3、EGF和EGFRmRNA的表达在正常口腔黏膜组织、不典型增生组织及癌组织中的表达率均依次增高,分别为25.0%(15/60)、40.0%(12/30)、68.3%(41/60)和33.3%(20/60)、43.3%(13/30)、80.0%(48/60)及18.3%(11/60)、46.7%(14/30)、75.0%(45/60),组间比较差异有统计学意义(P<0.05)。在口腔鳞癌组织中STAT3表达与癌的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05);EGF表达与癌的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05);EGFR表达与癌的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05)。口腔鳞癌中STAT3、EGF和EGFR mRNA的表达具有相关性。结论:STAT3、EGF和EGFR mRNA可能在口腔癌的浸润、转移及黏膜上皮癌变过程中起重要作用,STAT3、EGF和EGFR mRNA的联合检测可望成为口腔鳞癌早期诊断和判断预后的客观指标之一。
Objective：To investigate STAT3,EGF and EGFR mRNA in oral squamous cell carcinoma and its clinical significance.Methods：Using in situ hybridization,STAT3,EGF and EGFR gene expression were detected in 60 cases of oral squamous cell carcinoma,30cases of atypical hyperplasia and 60cases of normal oral mucosa.The results were statistically analysed in conjunction with the pathological grade,lymph node transferation of clinical indicators.Results：The expression of STAT3,EGF,and EGFR in the normal mucosa,dysplasia and carcinoma rates were getting higher gradually,which were 25.0%（15/60）,40.0%（12/30）,68.3%（41/60）and 33.3%（20/60）,43.3%（13/30）,80.0%（48/60）and 18.3%（11/60）,46.7%（14/30）,75.0%（45/60）respectively,showing a significant difference（P0.05）.In oral squamous cell carcinoma tissue,the STAT3,EGF and EGFR expression were closely related with the histological grade,depth of invasion and lymph node metastasis（P0.05）.The STAT3,EGF and EGFR gene expression in oral squamous cell carcinoma had correlated with each other.Conclusion：STAT3,EGF,and EGFR mRNA may be of great important in oral cancer invasion and metastasis and mucosal epithelial carcinogenesis.STAT3,EGF,and EGFR mRNA are expected to be one of the objective indicators of the diagnosis and prognosis of early oral squamous cell carcinoma.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2010年第4期547-550,共4页
Journal of Oral Science Research
基金
吉林省科委资助课题(编号:200705349)
