建立一种快速有效的大鼠腹腔心脏移植模型

Establishment of heterotopic abdominal heart transplantation model in rats

目的探讨一种快速而有效地建立大鼠腹腔异位心脏移植模型的方法。方法采用Ono术式并加以改良。供心摘取时,于心脏完全停跳后,在主动脉和肺动脉下过1/0丝线,一并结扎上、下腔静脉和肺静脉,提起丝线,同时剪断心脏各血管。实验组(Wistar-SD)和对照组(Wistar-Wistar)每组供、受体各20只。术后受体和移植心脏均存活72h视为手术成功,术后第4天两组供心组织行病理学观察。结果手术成功率为90%,供心摘取时间为(3.2±0.6)min。实验组移植心脏心肌组织呈急性排斥反应表现;对照组无急性排斥反应表现。结论该方法与目前应用较广泛的大鼠改进的Ono术式比较,取心时间明显缩短;实验组非同系(Wistar-SD)异位心脏移植复制出的大鼠供心排斥反应模型,可用于相关实验研究。

Objective To explore a quick and efficient surgical technique for establishing heterotopic abdominal heart transplantation model in rats. Methods Heterotopic abdominal heart transplantation of rats was performed by using Ono＇s method with modification. When donor heart stopped beating completely, the superior vena cava, inferior vena cava and pulmonary vein were ligated respectively with a 1/0 silk through aorta and pulmonary artery. After rai- sing the silk and cutting all blood vessels, the donor heart was harvested. There were 20 donors and 20 recipients in experimental group （Wistar to SD rats） and control group （Wistar to Wistar rats） respectively. Over 72 hour-survival of the animal after operation was considered a successful operation. On day 4, the transplanted hearts were observed histopathologically. Results The success rate of the operation was 90%. The donor heart harvesting time was（3. 2±0. 6） min. Acute rejection was observed in experiment group, and not in control group. Conclusion In this technique, the donor heart harvesting time is reduced significantly compared with the current widely used Ono＇s method. Wistar to SD rat heterotopic heart transplantation can establish the experimental animal model of immune rejection.