青心酮防治动脉粥样硬化与内脂素的关系

Preventive effect of 3,4-dihydroxyacetophenone on atherosclerosis and role of visfatin expression

目的:观察青心酮对ApoE(-/-)小鼠主动脉粥样硬化病变的影响与内脂素的关系。方法:取8只8周龄C57BL/6小鼠为正常对照组;取24只8周龄雄性ApoE(-/-)小鼠,随机分成3组:动脉硬化组(n=8,im,等量生理盐水);青心酮治疗组(n=8,im,10mg·kg-1·d-1);辛伐他汀治疗组(n=8,im,10mg·kg-1·d-1)。所有实验小鼠均饲以"西方类型膳食"饲料至12周。取血检测内脂素、血脂;剪取主动脉根部行冰冻切片,油红O染色观察主动脉粥样硬化病变情况;剪取主动脉根部斑块组织,观察斑块中内脂素的分布情况;行电镜切片,观察青心酮对斑块中平滑肌细胞、内皮细胞的结构影响。结果:青心酮治疗组血清内脂素减少,血脂降低,主动脉粥样硬化病灶形成减少,平滑肌细胞、内皮细胞损伤减轻,斑块中内脂素含量减少。结论:青心酮能减少ApoE(-/-)小鼠主动脉粥样斑块的形成,可能通过抑制内脂素生成而降低血脂发挥其作用。

AIM： To observe the preventive effect of 3,4-dihydroxyacetophenone （ DHAP） on atherosclerosis （ AS） and the role of visfatin expression in ApoE（-/-） mice. METHODS： Eight-week-old normal mice were used in normal control group （ n = 8） . Eight-week-old male ApoE （-/-） mice were randomly divided into 3 groups： AS group （ n = 8,im. NS） ,DHAP treatment group （ n = 8,im. DHAP 10 mg·kg-1·d-1） and simvastatin treatment group （ n =8,im. simvastatin 10 mg·kg-1·d-1） . All mice were fed with Western diet （ 21% fat,0. 15% cholesterol） for 12 weeks. The blood samples were collected and the concentrations of blood lipids and visfatin were detected. The frozen sections of aortic root were stained with oil red O. The visfatin in atherosclerotic plaques at aortic roots was examined by Western blotting. The structures of smooth muscle cells and endothelial cells were observed under electron microscope. RESULTS： In DHAP-treated mice,the concentrations of visfatin,TG and TC were decreased,the formation of AS plaque was reduced,the injuries of smooth muscle cells and endothelia cells were attenuated. Visfatin was also decreased at atherosclerosis plaque in DHAP-treated mice. CONCLUSION： DHAP effectively prevents and treats AS by inhibiting the production of visfatin and reducing lipid.