重组人睫状神经营养因子对大鼠坐骨神经再生长的影响

The effects of ciliary neurotrophic factor on rat sciatic nerve regeneration

目的:观察重组人睫状神经营养因子(CNTF)对大鼠坐骨神经再生长的影响,并与神经生长因子(NGF)的作用进行比较。方法:随机将动物分成正常对照组、模型组、CNTF给药小剂量组(48μg/kg)、中剂量组(216μg/kg)、大剂量组(1080μg/kg)、NGF给药组(20μg/kg),每组各10只。模型组采用大鼠坐骨神经切断再缝合法造成坐骨神经损伤模型,给药组采用不同药物剂量对神经损伤部位肌肉注射给药45d后,测定神经动作电位潜伏期和传导速度。结果:与模型组相比较,CNTF各给药组神经动作电位潜伏期显著缩短(P<0.01),传导速度显著增快(P<0.01);同时,216μg/kg、1080μg/kgCNTF组和NGF组对神经动作电位潜伏期的影响无显著差别(P>0.05),但是216μg/kg、1080μg/kgCNTF组的神经动作电位传导速度比NGF组显著增快(P<0.01)。结论:CNTF对大鼠坐骨神经再生具有一定的促进作用,而且其作用可能优于NGF。

AIM： To observe the effects of ciliary neurotrophic factor （ CNTF） on rat sciatic nerve regeneration,and compare the similarities and differences of its effects with that of nerve growth factor （ NGF） . METHODS： Sciatic nerve impairment model was prepared by break-restoring rat sciatic nerve. After intramuscular injection of different doses of CNTF at the damaged nerve for 45 days,the latent period and conduction velocity of action potential were determined. RESULTS： Compared with model group,the latent periods of all the CNTF treatment groups were significantly shortened （ P 0. 01） and the conduction velocity became faster significantly （ P 0. 01） . Additionally,the effect on latent period in middle and high CNTF dosage groups is not significantly different form that in NGF treatment group （ P 0. 05） . However,the conduction velocity in CNTF high dosage group is significantly faster than that in NGF treatment group （ P 0. 01） . CONCLUSION： The effect of CNTF on promoting the rat sciatic nerve regeneration is better than that of NGF.