蜂胶对小鼠体内胆固醇逆转运的影响

Effect of Propolis on Reverse Cholesterol Transport in Mice in Vivo

目的观察蜂胶干预后小鼠血浆、肝脏、粪便、肾上腺中3H-胆固醇的分布和血浆脂质水平的变化,探讨蜂胶对小鼠体内胆固醇逆转运的影响。方法 14只C57BL/6小鼠随机分为对照组和蜂胶组,分别给予单纯溶剂和蜂胶灌胃4周后,腹腔注射经乙酰化低密度脂蛋白及3H-胆固醇处理过的小鼠巨噬细胞悬液,单独笼养48 h,取血,留置肝脏和肾上腺,收集粪便。酶法测定血浆脂质水平;经液体闪烁计数测定血浆、肝脏、粪便、肾上腺中的3H-胆固醇含量。结果与对照组相比,蜂胶升高血浆高密度脂蛋白胆固醇和总胆固醇水平分别为39%和29%,对低密度脂蛋白-胆固醇和甘油三酯的水平影响不大;注入细胞后血浆3H-胆固醇分布明显的迅速增高,6 h为对照组2.9倍,24 h为对照组1.7倍;肝脏中3H-胆固醇分布降低了39%;粪便中的3H-胆固醇分布降低了60%;而小鼠肾上腺中3H-胆固醇的分布升高了84%。结论蜂胶在体内促进了外周巨噬细胞中多余的胆固醇的流出和肾上腺组织的利用。

Aim To explore the effect of propolis on reverse cholesterol transport in mice in vivo. Methods Fourteen C57BL/6 male mice were divided into 2 groups,which were treated with either vehicle or propolis.After 4 weeks,Mice were bled from the retro-orbital plexus and plasmas lipid profiles were determined by enzymatic method.Then mice were injected intraperitoneally with 3H-cholesterol–labeled and cholesterol-loaded macrophages（6×106 cells containing 5.9×105 cpm in 0.5 mL medium） and monitored for the appearance of 3H-tracer in plasma,liver,feces and adrenal. Results In contrast to the control,the levels of high-density lipoprotein cholesterol（HDLC） and total cholesterol（TC）in plasmas of the propolis group were increased by 39% and 29%,while the levels of low-density lipoprotein cholesterol（LDLC） and triglyceride（TG） had no significant changes.After intraperitoneally injected,the appearance of 3H-tracer in plasma of propolis group was rapid,with a 2.9-fold increase at 6 hours and a 1.7-fold increase at 24 hours compared with the control group.The appearance of 3H-tracer in the liver and feces were decreased by 39% and 60%,respectively.The appearance of 3H-tracer in the two adrenals was increased apparently by 84%. Conclusion Propolis can promote cholesterol efflux from the peripheric macrophages and utilization in the adrenals in mice in vivo.