替米沙坦联合阿托伐他汀对糖尿病大鼠内皮细胞形态、功能的影响

Telmisartan and Atorvastatin's Association Influence on the Vascular Endothelial Cell's Morphology and Function in Diabetes Rats

目的观察替米沙坦联合阿托伐他汀对糖尿病大鼠血管肝细胞生长因子表达及内皮细胞形态的影响,为临床防治糖尿病早期动脉粥样硬化提供实验依据。方法建立糖尿病大鼠模型并干预,检测各组血管肝细胞生长因子的表达以及血管内皮细胞的形态变化,各组之间进行比较。结果与正常对照组比较,糖尿病对照组血管肝细胞生长因子蛋白及肝细胞生长因子mRNA表达明显减弱;透射电镜下血管内皮细胞发生了坏死、脱落等改变,内皮功能受损。与糖尿病对照组比较,替米沙坦组血管肝细胞生长因子蛋白及肝细胞生长因子mRNA表达显著增强;透射电镜下血管内皮细胞形态明显改善。与替米沙坦组比较,联合干预组血管肝细胞生长因子蛋白及肝细胞生长因子mRNA表达无显著变化;透射电镜下血管内皮细胞形态改善更加明显。结论替米沙坦联合阿托伐他汀能更好地改善糖尿病大鼠血管内皮细胞的形态、功能,在临床防治糖尿病早期动脉粥样硬化中值得进一步研究。

Aim To observe telmisartan and atorvastatin＇s association influence on the expression of hepatocyte growth factor of blood vessel and the vascular endothelial cell＇s morphology and function in diabetes rats which can supply with laboratory material for preventing and caring artherosclerosis of patients with diabetes in early stage during clinic practice. Methods Animal model of the diabetes rats were built and divided into different groups which were given different medicine intervention,and the expression of hepatocyte growth factor was tested and the morphology of endothelium within each group was observed,then the results of each group were compared. Results Compare to normal group,the diabetes groups manifested lower concentration of hepatocyte growth factor,mRNA expression of hepatocyte growth factor decreased and the endothelium＇s necrosis falling off from blood vessel were observed under transmission electron microscope.Compared to pure diabetes group,the groups with medicine intervention,either single telmisartan or telmisartan associated with atorvastatin,both had higher hepatocyte growth factor concentration level and hepatocyte growth factor mRNA expression,the morphy of blood vessel endothelium become much better and normalizing.Compared to single telmisartan group,the hepatocyte growth factor concentration and hepatocyte growth factor mRNA expression were much higher in the group of telmisartan associated with atorvastatin,the morphology of endothelium was more normalizing. Conclusion Telmisartan associated with atorvastatin can improve the morphology of endothelium better than single telmisartan,which is worthy of the further research in the clinical prevention and treatment of atherosclerosis of patients with diabetes in early stage.