摘要
目的:观察慢性低灌注大鼠血-脑脊液屏障(BBB)通透性的变化及丁苯肽对其通透性的影响。方法:Wistar大鼠144只,双侧颈总动脉结扎方法制作慢性低灌注模型,随机分为假手术组,缺血组和丁苯肽组,各48只,于术后7、14、306、0 d(每时间点12只)比较各组大鼠脑组织BBB通透性。结果:伊文思蓝染色显示缺血组7 d亚组大鼠脑组织伊文思蓝含量显著高于假手术组(P<0.01),高于丁苯肽组(P<0.05),缺血组14 d亚组大鼠脑组织伊文思蓝含量高于假手术组和丁苯肽组(P<0.05),30 d、60 d亚组大鼠脑组织伊文思蓝含量在各组间差异无统计学意义。结论:慢性低灌注状态下,大鼠的BBB通透性增高,丁苯肽可缓解BBB通透性的增高。
Objective： To investigate the changes in permeability of blood-brain barrier（BBB） in rats with chronic hypoperfusion and the protective effect of butylphthalide on it.Methods： Rat models of chronic hypoperfusion were established by ligating the bilateral carotid arteries.One hundred and forty-four rats were randomly divided into control group,ischemic group and butylphthalide treatment group.At days 7,14,30 and 60 after operation,the permeability of BBB was evaluated by the Evans blue staining.Results： At day 7 the permeability of BBB of rats in ischemic group significantly increased compared with that in control group（P0.01） and butylphthalide treatment group（P0.05）.At day 14 the permeability of BBB of rats in ischemic group significantly increased compared with that in both control group and butylphthalide treatment group（P0.05）.There is no significant difference at day 30 and 60 among the three groups.Conclusion： The permeability of BBB is increased in rats with chronic hypoperfusion and butylphthalide could lessen the destruction of BBB.
出处
《神经损伤与功能重建》
2010年第5期335-337,354,共4页
Neural Injury and Functional Reconstruction
关键词
慢性低灌注
血-脑脊液屏障
丁苯肽
chronic cerebral hypoperfusion
blood-brain barrier
butylphthalide
