水产品中河鲀毒素的高效液相紫外测定法

Detection of tetrodotoxin by HPLC-UV in aquatic product

以鲀科鱼类为研究材料,采用超声波法提取,C18-WCX联用固相萃取纯化,使用高效液相色谱紫外检测方法测定水产品中河鲀毒素含量,通过探索提取方法和色谱条件对检测结果的影响,并确定和优化色谱分离和紫外检测条件,建立了一种高效液相紫外检测测定水产品中河鲀毒素的方法。该检测方法稳定性好,可操作性强,加标回收率在75%～82%之间,相对标准偏差小于15%,检出限达到0.02mg/kg。与现行河鲀毒素检测方法相比,该方法具有对仪器要求低、操作简便的特点,易于推广应用。

Occurrence of poisoning caused by tetrodotoxi（nTTX）is frequently reported in recent years.The establishment of a good detection method should be helpful to promote the monitoring work of TTX in aquatic product.Impact of extraction method and chromatographic conditions on TTX detection were studied,and a stable,cost-effective detection method was developed in this study.Puffer fishes were selected as the main research material.After ultrasonic extraction,the extracted solution were purified by C18 and WCX solid-phase extraction cartridge in sequence,then they were separated by reverse phase ion-pair liquid chromatography and detected by UV-detector.The recovery rates were 75%-82%.Relative standard deviations（RSDs）were less than 15%,and the detection limit was 0.02mg/kg.The stability,sensitivity and operability of this method is better than other detection methods,and its application would be carried out more easily.