无线遥测温度梯度仪的研制及性能验证

Exploitation of Radiotelemetric Temperature Gradient Apparatus and its Function Characterization Virification

目的研制无线遥测温度梯度仪及性能的验证。方法我们利用行为性体温调节反应的原理,研制无线遥测温度梯度仪。实验将大鼠置于温度梯度箱内允许选择环境温度的范围为15～40℃,并且动物可以在箱内自由运动。光电探测器监测动物在梯度箱内所处的位置,同时用遥测系统测量体核温度。结果 (1)注射脂多糖(LPS)后大鼠的体核温度和选择环境温度均明显高于盐水组。(2)注射精氨酸加压素(AVP)后可导致体核温度快速降低。在给药30min后,体温从37.4℃降低到35.7℃,同时动物选择环境温度降低4℃。注射盐水对体核温度和动物选择环境温度几乎没有影响。结论我们研制的无线遥测温度梯度仪可以作为监测大鼠和小鼠行为性体温调节反应的可靠仪器。另外,行为性体温调节观察表明,AVP能降低体温调定点,因为在AVP引起低温期时,大鼠选择冷环境温度区。

Objective To produce radiotelemetric temperature gradient apparatus and verify its function characterization. Methods By applying the principle of behavioral thermoregulatory responses, we developed a radiotelemetric temperature gradient apparatus. The rat was placed in a temperature gradient that permitted the selection of ambient temperature （Ta） ranging from 15℃ to 40℃. A rat moved freely in the chamber. Its location was monitored photoelectrically while its core temperature was monitored by a telemetry system. Results （ 1 ） LPS injection caused significant increase in core temperature （To） and selection of Ta as compared to saline administration. （2） Arginine vasopressin （ AVP ） led to a rapid reduction in Tc from 37.4 to 35.7℃ within 30min after injection. There was a 4℃ reduction in Ta selected by rats within 30min after injection simultaneously. Administration of saline had little effect on Tc and selected Ta. Conclusion Our radiotelemetric temperature gradient apparatus can be a reliable method for continuous monitoring behavioral thermoregulatory responses of rats and mice. In addition, behavioral thermoregulatory observations suggest that the AVP can decrease the temperature set point, because rats treated with AVP selected cooler Ta＇s during the period of AVP - induced hypothermia.