HIV-1感染者不同人群的亚型分析

Analysis on Subtypes in Different Groups of HIV-1 Infectors

目的研究河北省2008年新确认的HIV-1感染者不同人群的亚型分布情况。方法选取2008年新确认的HIV-1感染者,利用分子生物学方法进行分型后,对不同人群的亚型分布情况进行分析,探讨不同HIV-1感染者人群的亚型分布比例有无差异。结果在58例已确定亚型的HIV-1感染者中,来源于性传播、吸毒、血液和不详4种不同的人群,包含了B、CRF01-AE、CRF07-BC、CRF08-BC和C5种亚型。在这4种人群中,性传播途径的人群以B亚型(42.1%)和CRF01-AE(34.2%)为主,但是5种亚型的毒株均存在;血液传播途径中,均为B亚型;在吸毒人群中,均为CRF07-BC亚型;不详人群中亚型相对较复杂,包含了C亚型除外4种亚型。结论河北省HIV感染者亚型的分布具有明显的人群特点。

Objective To study the subtypes distribution of HIV - 1 newly infected individuals in different groups in Hebei Province during 2008. Methods We collected blood of 63 HIV - 1 newly infected individuals and separated plasma specimens from the blood samples. RNAs were extracted and amplified by RT - PCR and nest - PCR. The PCR products were sequenced directly and phylogenetie analysis was performed. The subtypes distribution was studied in different groups. Results Among the 58 cases of HIV - 1 infections who were diagnosed with the subtypes, there were 5 subtypes including B,CRFOI_AE,CRF07 -BC,CRF08 -BC and C in 4 infected groups through sex transmit, drug - taking, blood transfusion and some unknown reason. The infected group through sex transmit mainly consisted of subtype B （42.1% ） and CRF01_AE（34.2% ） but the five subtypes of virus all existed. Those infected through blood transfusion were mainly B,and those of drug takings were all with CRF07 BC. Among the rest, subtypes were relatively complex, containing four subtypes except subtype C. Conclusion The subtypes distribution of HIV - 1 newly infector in Hebei province had significant characteristic in different groups.