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MAGE融合疫苗的构建与表达

Construction and expression of MAGE combinative vaccine
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摘要 目的:构建MAGE1和MAGE3融合表达载体,并获得抗血清。方法:采用分步交叉亚克隆方法将两个不同片段连接于表达载体,分组对照优化表达条件,用SDS-PAGE和Western-blot检测表达与纯化结果,将纯化蛋白免疫动物后用琼脂双扩和ELISA方法检测抗血清效价。结果:成功构建了MAGE1-MAGE3表达载体并获得纯化蛋白;免疫动物后获得高效价的抗血清。结论:得到纯度高,变性度低的融合蛋白。 Objective：To construct combinant MAGE1 and MAGE3 expression vector and acquirement of rabbit anti-serum of combinant protein.Methods：To construct expression vector by truncated sub-clone and cross clone; verified by SDS-PAGE and Western-blot; agar precipitation and ELISA detected the titer of anti-serum.Results：Successfully constructed the expression vector on pET-28a.Conclusion：Acquired the optimal expression conditions and combinant protein,and obtained high titer anti-serum.
作者 陈连宏 马宁强 王军伟 张志培
机构地区 第四军医大学唐都医院肿瘤科
出处 《现代肿瘤医学》 CAS 2010年第9期1705-1707,共3页 Journal of Modern Oncology
关键词 黑色素瘤抗原 原核表达 肿瘤 MAGE prokaryotic expression tumor
分类号 R730.51 [医药卫生—肿瘤]
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参考文献7

  • 1Ye J,Chen GS,Song HP,et al.Heat shock protein 70 / MAGE-Ⅰ tumor vaccine can enhance the potency of MAGE-1-specific cellular immune responses in vivo[J].Cancer Immunol Immtmother,2004,53(9):825-834.
  • 2Ma JH,Sui YF,Ye J,et al.Heat shock protein 70/MAGE -3 fusion protein vaccine can enhance cellular and humoral immune responses to MAGE-3 in vivo[J].Cancer Immunol Immunother,2005,54(9):907-914.
  • 3张伟东,苗树军.我国恶性肿瘤死亡率流行病学特征分析[J].中国健康教育,2009,25(4):246-248. 被引量：139
  • 4张澎,于津浦,安秀梅,任秀宝,郝希山.肺癌患者中MAGE的表达及临床意义[J].检验医学,2004,19(3):232-234. 被引量：4
  • 5Kim HR,Kim TH,Chung JH,et al.The detection of peripheral lung cancer by MACE A1 -6 RT-nosted PCR in bronchial washing specimens[J].Lung Cancer,2009,65(2):166-169.
  • 6Liu W,Cheng S,Asa SL,et al.The melanoma-associated antigen A3 mediates fibronectin-controlled cancer progression and metastasis[J].Cancer Res,2008,68(19):8104-8112.
  • 7Grah J,Samija M,Juretié A,et al.Immunohistochemical expression of cancer/testis antigens (MAGE-A3/4,NY-ESO-1) in non-small cell lung cancer:the relationship with clinical-pathological features[J].Coll Antropol,2008,32(3):731-736.

二级参考文献17

  • 1World Health Organization. Cancer incidence, mortality and survival by site for 14 regions of the world [ R]. Geneva:Global Programme on Evidence for Health Policy Discussion Paper,2001.
  • 2卫生部.全国健康教育与健康促进工作规划纲要(1005-2010)[R].北京:卫生部,2004.
  • 3World Health Organization. World cancer report [ R]. Geneva: WHO, 2003.
  • 4Tonani M, Carvalho EC. Cancer risk and preventive behavior: persuasion as an intervention strategy [ J ]. Rev Lat Am Enfermagem. 2008,16(5) :864 -870.
  • 5Frieden TR, Myers JE, Krauskopf MS. A public health approach to winning the war against cancer[ J]. Oncologist,2008,17 : 17.
  • 6Mori M, Inoue H, Mimori K, et al. Expression of MAGE genes in human colorectal carcinoma[J]. Ann Surg, 1996,224:183-188.
  • 7Inoue H, Honda M, Li J, et al. The expression of tumor-rejection antigen''MAGE'' genes in human gastric carcinoma[J]. Gastroenterology,1995,109:1522-1525.
  • 8Weynants P. Expression of MAGE genes by non-small-cell lung carcinomas[J]. Int J Cancer,1994,56:826-829.
  • 9Yoshimatsu T. Expression of the melanoma antigen-encoding gene in human lung cancer[J]. J Surg Oncol,1998,67:126-129.
  • 10Shichijo S, Hayashi A, Jakamori S, et al. Detection of MAGE-4 protein in lung cancer[J]. Int J Cancer,1995,64:158-165.

共引文献141

现代肿瘤医学
2010年 第9期

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