恶性肿瘤患者化疗前后的淋巴细胞亚群分析

Analysis of lymphocytes subsets before and after chemotherapy in patients with malignant tumors

目的:观察恶性肿瘤患者化疗前后淋巴细胞亚群的变化特征,探讨恶性肿瘤患者的机体免疫状况。方法:采用流式细胞术对100例住院恶性肿瘤患者的淋巴细胞亚群进行分析,观察患者化疗前后的淋巴细胞亚群的变化特征。同时收集30名健康体检者作为正常对照。结果:恶性肿瘤患者CD8+细胞、B细胞高于正常,而CD3+细胞、CD4+细胞、NK细胞及CD4+/CD8+比值显著低于正常水平(P<0.05);化疗后完全缓解组以上4项趋于正常(P>0.05);而未缓解组与完全缓解组及正常对照组相比有明显差异(P<0.05)。结论:恶性肿瘤患者细胞免疫功能衰退,体液免疫功能略增高,化疗后缓解的病人各指标趋于正常,与病情发展一致,淋巴细胞亚群分析可作为监测恶性肿瘤患者免疫状态的指标。

Objective：To study the change of lymphocyte subsets before and after chemotherapy in malignant tumor. Methods： The Lymphocyte subsets before and after chemotherapy of 100 patients with malignant tumors were detected by flow cytometry . And 30 healthy people were selected as normal control. Results： Before chemotherapy, CD^8＋ and B cell level in the patients were significantly higher than controls （P〈0.05）. On the contrary, CD^3＋, CD^4＋,NK, and CD^4＋ /CD^8＋ratio in patients were significantly lower than those in controls （P〈0.05）. After chemotherapy, the value in the patients were not much different from those in controls, while values in the remaining 66 patients were still significantly different from those in controls （P〈0.05）. Conclusion： Cell immunity are impaired in patients with malignant tumors, but the humoral immune function enhanced. After chemotherapy, the value in the patients were not much different from normal, it is coincidence with the progression of disease. lymphocyte subsets analysis is a good value to detect the immune state in patients with malignant tumors.