SSX_2基因在急性白血病中表达的临床意义

Expression of SSX_2 gene and its clinical significance in acute leukemia

目的:研究滑膜肉瘤X断裂点基因2(SSX2)在急性白血病中的表达及临床意义。方法:逆转录聚合酶链反应检测72例急性白血病(AL)中SSX2的表达,包括23例急性淋巴细胞白血病(ALL)及急性髓细胞白血病(AML)。结果:在72例AL患者中有38例SSX2表达阳性(52.78%),在ALL中SSX2表达阳性率为56.52%,而在AML中SSX2表达阳性率为51.02%,两者之间没有显著性差别(P<0.05)。在非M3型AML患者中SSX2表达阳性的完全缓解率(CR)为60.87%,而在SSX2表达阴性的完全缓解率为89.47%。在随访的16例SSX2表达阳性患者观察治疗后的SSX2表达阳性率,完全缓解后SSX2表达可以转为阴性,SSX2表达持续阳性的患者会较早复发。如果患者没有完全缓解,则SSX2表达不会转为阴性。结论:在AML及ALL患者中SSX2表达没有差别(P>0.05),SSX2阳性表达非M3型AML患者中CR率较低,SSX2可以作为AML患者较差的预后指标。

Objective：To study the expression of SSX2 gene and its clinical significance in acute leukemia.Methods： Reverse transcriptase polymerase chain reaction（RT-PCR）was performed to detect expression of SSX2 in 72 samples of acute leukemia（AL） including 23 acute lymphoblastic leukemia（ALL） and 49 acute myelogenous leukemia （AML）.The relationship between SSX2 expression and response were analyzed.Results： The expression of SSX2 was positive in 38 of 72 AL patients （52.78%）.The positive rates of SSX2 expression were 56.52% in ALL and 51.02% in AML,with no statistically significant diference. The complete remission（CR）rates were 60.87% in SSX2 positive non-M3 AML patients and 89.47% in SSX2-negative non-M3 AML patient .16 patients with positive SSX2 expression were followed up to observe the changes of SSX2 expression after treatment.The SSX2 could turn negative after getting CR.The patients who had gotten CR could relapse earlier when SSX2 was continent positive. SSX2 positive expression did not turn negative when the patients did not get CR.Conclusion： The positive expression of SSX2 is similar between AML and ALL.The non-M3 AML patients with positive SSX2 expression got lower CR rate, which indicates that SSX2 can be used as a poor prognostic factor in AML.Monitoring of the SSX2 expression may be a useful means for the detection of minimal residual disease in AL.