用羰花青荧光染料追踪家鸽顶盖与中脑核团的神经连接

APPLICATION OF A CARBOCYANINE FLUORESCENT DYE TO TRACING NEURONAL CONNECTIONS OF THE PIGEON TECTUM WITH SOME MESENCEPHALIC NUCLEI

本文用亲脂质荧光染羰花青(Carbocyanine)的衍生物——1.1’一二(十八烷基)—3,3,3,’3’—四甲基吲哚羰花青高氯酸盐(1,1’,dioctadecyl—3,3,3’,3’—tetramethyli-ndocarbocyanine perchlorate,dil),追踪了家鸽(Columba livia)顶盖深层与中脑核团的神经连接.结果表明,顶盖深层接收峡核大细胞部(Nucleus isthmi pars magnocellularis,Imc)以及动眼神核腹部(Nucleus nervi oculomotorii,pars ventralis,OMv)中脑外侧核背部(Nucleus mesencephalicus lateralis,pars dorsalis,MLd)的神经投射,本文还对这些结果与以前用类霍乱原-HRP(CB HRP)方法所得结果进行了比较分析.

In this study, diI (1, 1, dioctadecyl-3, 3, 3', 3'-tetramethyl-indocarbocyanine perchlorate) as a derivative of the carbocyanine dye, a fluorescent lipophilic substance, was applied in fixed tissue for tracing the neuronal connections of the deep layers of the tectum with some mesencephalic nuclei in pigeons (Columba livia). The results indicate that the deep layers (II h - III ) of the tectum receive their neuronal inputs from the nucleus isthmi pars magnocellularis (Imc), the nucleus nervi oculomotorii, pars ventralis (OMv) and the nucleus mesencephalicus lateralis, pars dorsalis (MLd). A comparative analysis has been earned out between the results of this study and those obtained with CB-HRP.