摘要
本文采用脉冲激光荧光技术及常规的荧光分光光度计详细地研究了顺铂对DNA变性温度的影响,DNA氢键受损的碱基对数随温度变化,DNA的G—C碱基含量对顺铂作用效果以及顺铂浓度的影响,初步探讨了抗癌药物顺铂对DNA的结构影响.
Cis-diamminedchloroplatinum( II ) (cis-DDP) is a kind of antitumous medicine. It bonds to the N-7 of the guanine of DNA and causes intrastrand or intrastrand cross - linking. Our experiments induce fluorecence techniques to analyze the influence of cis-DDP on DNA denaturation so as to give some information on the interaction between cis-DDP and DNA. The results are as fellows:(1) proved that as - DDP made hydrogen bond of G - C easy to be damaged in vitro.(2) Got the percent of damaged hydrogen bonding in DNA at different temperature befor and after cis-DDP was added(3) the influence of the percent of G-C base in DNA.(4) the influence of the concentration of cis-DDP(5) the brief discussion of the mechanism of antitumor
出处
《生物物理学报》
CAS
CSCD
北大核心
1990年第2期275-280,共6页
Acta Biophysica Sinica
