三七对酒精性肝病大鼠肝组织NF-κB、c-Jun表达的影响

Effects of Notoginseng on Hepatic Expression of Nuclear Factor-κB and c-Jun in Alcoholic Hepatopathy Rats

目的观察三七对酒精性肝病大鼠肝组织NF-κB、c-Jun表达的影响。方法SD雄性大鼠连续14周给予酒精建立酒精性肝病模型,随机分为模型对照组,三七高、低剂量组和硫普罗宁组,在模型制备同时,每天下午分别灌服给药,连续14周。同时设立正常对照组,连续14周灌胃给水。免疫组化法检测肝组织中NF-κBp65/IκBα、p-IκBα、p-JNK、c-Jun蛋白的表达。结果模型对照组大鼠肝组织NF-κBp65/IκBα、p-IκBα、p-JNK/c-Jun均较正常对照组明显升高(P<0.01);三七高、低剂量组大鼠肝组织NF-κBp65/IκBα、p-IκBα、p-JNK/c-Jun表达较模型对照组明显降低(P<0.01,P<0.05)。结论三七能显著抑制肝组织中NF-κBp65/IκBα、p-JNK/c-Jun的过度表达,这可能是其有效防治酒精性肝病发生发展的重要机制之一。

OBJECTIVE To investigate the effects of notoginseng on the expression of nuclear factor-κB and c-Jun in hepatic tissues of alcoholic hepatopathy rats.METHODS SD male rats were induced alcoholic hepatopathy in 14 weeks and randomly divided into normal group,model group,high-dose notoginseng group,low-dose notoginseng group and tiopronin group.All rats were treated every afternoon during the 14-week respectively.The normal group was treated with water during the 14-week.Protein expression of NF-κBp65/IκBα,p-IκBα and p-JNK/c-Jun in the liver was measured by two-step immunohistochemistry.RESULTS NF-κBp65/IκBα,p-IκBα and p-JNK/c-Jun expression were significantly elevated in alcoholic hepatopathy rats（P0.01）.High-dose and low-dose of notoginseng both reduced NF-κBp65/IκBα and p-JNK/c-Jun expression relative to untreated alcoholic hepatopathy rats（P0.01,P0.05）.CONCLUSION Notoginseng can inhibit the overexpression of NF-κBp65/IκBα and p-JNK/c-Jun in hepatic tissue.This may serve as one of the critical mechanisms through which notoginseng efficiently prevents alcoholic liver diseases.