摘要
借助位移试剂(Shift Reagent)测细胞内外阳离子浓度的NMR方法是近十年发展的新技术,可直接观测完整活细胞和组织内外的阳离子浓度,具有非破坏性连续性观察的优点.本实验室用自己制备的位移试剂测试NaCl溶液,人红细胞和豚鼠红细胞内Na^+浓度.获得良好的^(23)Na NMR谱的共振分离.
NMR in combination with an anionic paramagnetic shift reagent ( SR) has been used to study intracellular and extracellular cation concentrations. It is a new developing technique recently. The SR has made it possible to observe the intra- and extracellular Na+ and K+. NMR has the advantages over conventional techniques such as flame photometry and radioisotope tracer of being nondestructive, noninvasive and continuous, so that net fluxes can be followed with a reasonably fast time resolution concentrations. Avoiding possible artifacts of the isolation precedures. We have got some primary results in RBC by NMR with shift reagent.
出处
《生物物理学报》
CAS
CSCD
北大核心
1990年第4期476-479,共4页
Acta Biophysica Sinica