半边旗提取物5F-Na盐溶液对三种肝细胞癌细胞株的增殖抑制作用

Inhibition of Proliferation by 5F-Na Solutions from Pteris Semipinnata L in Hepatocellular Carcinoma Cell Lines

目的:评估半边旗提取物5F-Na盐溶液对肝细胞癌细胞株的增殖抑制作用。方法:采用MTT法,观察不同浓度的半边旗提取物物5F-Na盐溶液分别作24、48、72h对三种不同类型肝细胞癌细胞株(HepG2,Hep3B,BEL-7402)生长增殖的影响。结果:不同浓度的半边旗提取物5F-Na盐溶液分别对3种肝癌细胞作用24、48、72h后,所有的肝癌细胞株生长增殖均受到不同程度的抑制(P<0.05),5F-Na盐溶液对HepG2,Hep3B,BEL-7402作用72h后的IC50分别是20.67,16.74,16.09mg/L。结论:半边旗提取物5F-Na盐溶液能有效抑制肝细胞癌细胞株的体外增殖。

Objective：To evaluate the inhibitory effect of Psl5F-Na solutions on proliferation of hepatocellular carcinoma cell lines in vitro. Methods：After treatment of hepatocellular carcinoma（ HCC） cell lines（ HepG2,Hep3B,BEL-7402） with various concentration Psl5F -Na solutions for 24,48,and 72 hours,the proliferation capacity of cells was observed by MTT assay. Results：After treated with Psl5F-Na solutions,the proliferation of these hepatocellular carcinoma cell lines were inhibited differently（ P 0. 05）. The IC50 for 72h treatment of HepG2,Hep3B,BEL-7402 were 20. 67,16. 74,16. 09mg/L respectively. Conclusion：Psl5F-Na solutions could inhibit the proliferation of hepatocellular carcinoma cell lines in vitro effectively.