胃肠舒片对胃肠功能障碍大鼠乙酰胆碱的影响

Study on the Effect of Weichangshu Tablet on Ach in Rat with Gastrointestinal Dynamics

目的:探讨胃肠舒促进胃肠动力的作用机制。方法:将50只Wister大鼠随机分为正常对照组、模型对照组、胃肠舒小剂量组、胃肠舒大剂量组、西沙必利组。除正常对照组外,其余四组均制备胃肠动力障碍动物模型。各组用等容积的受试药液灌胃,连续7天,将各组动物麻醉后选取胃肠标本。免疫组化法观察神经递质Ach的表达变化,图像分析仪测定其肌间神经丛光密度值。结果:胃肠舒组及西沙必利组均能明显升高胃肠组织中AchE含量,与模型组比较有显著差异(P<0.01),胃肠舒大剂量组升高胃窦中AchE含量较西沙必利组显著(P<0.01)。结论:通过增加胃肠道Ach分泌,Ach作用于平滑肌M受体,开放质膜上L型Ca2+通道,使Ca2+内流而加强胃肠平滑肌收缩。

Objective：Investigating the effect of Weichangshu on gastrointestinal motility. Methods：Divide 50 Wistar rats in to 5 groups randomly：normal control,model control,low dosage of Weichangshu,high dosage of Weichangshu,Cisapride,10 in each group. In addition to the normal control group,the other four groups were prepared animal models of gastrointestinal motility disorder; the groups were given the same volume solution of medicine by way of gastric perfusion. for eleven days,samples were taken from antrum and campylobacter after animals were anesthetized. Achwere showed with immunohistocheinical method by immunohistocheinical method. The optical density of myenteric plexues were measured with mitrosperegraph. Results：Groups of Weichang-shu and Cisapride can effectively increase AchE in gastro-intestinal tract compared with model control（ P 0. 01）. Compared with Cisapride ,high dosage of Weichangshu Tablet can effectively increase Ach in antrum（ P 0. 01）. Conclusion：It can increase Ach ,which act on M-receptor and opens L Ca2 ＋ channel on protoplasmic membrane and cause gastrointestinal smooth muscle contraction.