土壤中降解聚己内酯(PCL)微生物菌种的筛选及降解性能

STUDY ON THE PCL-DEGRADING MICROORGANISMS IN GENERAL SOIL AND THEIR DEGRADATION PROPERTIES

为了获得土壤中能降解聚己内酯(PCL)的微生物并研究其降解效果,对陕西普通土壤中的细菌、真菌、霉菌和放线菌进行驯化、筛选,并分别对PCL进行生物降解,研究降解前后PCL质量损失、表面形态、结晶度三方面的变化,结果表明,在陕西普通土壤中存在能够降解PCL的细菌、真菌、放线菌,其中放线菌的降解能力最好,真菌中霉菌的降解能力相对较弱,部分细菌具有较好的降解能力.经生理生化实验鉴定:具有较强降解PCL的细菌为蜡状芽孢杆菌.经上述菌种侵蚀后的PCL球晶均受到一定程度的破坏,其结晶度都有所降低.

To obtain the microorganism from soil which can degrade PCL and to investigate their degradation abilities,we domesticated and screeed the microorganisms from Shaanxi general soil including bacteria,fungi mold and actinomycete.By comparing the mass loss,surface mophology and crystallinity between nature and degraded PCL,PCL-degrading bacteria,fungi and actinomycete were found to exist in Shaanxi general soil.Among these microorganisms,actinomycetes have the strongest degradation ability,while the ability of fungi is comparably weak,and some bacteria have good degradation ability.According to the biological and biochemical experiments,the bacteria that can degrade PCL effectively are Bacillus cereus.After the erosion by the above microorganisms,the spherulites of PCL were destroyed to some content,the crystallinity reduced correspondingly.